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目的:探讨125I-UdR提高内皮抑素基因对大鼠种植癌模型的抑制效应。方法:制作肿瘤株Walker-256细胞大鼠皮下种植癌的动物模型并分为1-4组(对照组、125I-UdR组、Endostatin组和Endostatin+125I-UdR组),每组20只,通过实体瘤内注射,分别给与相同体积生理盐水125I-UdR、内皮抑素基因及125I-UdR和内皮抑素基因混合物,测量肿瘤治疗前体积(V0)和治疗后不同时间体积(Vt),计算10d、20d的肿瘤生长率(f=Vt/V0),观察各组肿瘤在光镜下的变化。结果:各组大鼠10d、20d肿瘤生长率为:(11.03±1.08、27.35±1.08),(4.02±0.79、7.58±2.98),(3.88±0.26、7.02±2.75),(2.72±1.01、2.94±1.26),2、3、4组的肿瘤生长率明显小于1组(P<0.001);2、3组之间肿瘤生长率差别不明显(P>0.05),4组肿瘤生长率小于2、3组(P<0.01)。结论:通过实体瘤内注射的方法给与125I-UdR、内皮抑素基因及两者混合物后,能够明显抑制肿瘤的生长,内皮抑素基因和125I-UdR联合治疗在抑制肿瘤生长方面作用更加显著。
Objective: To investigate the inhibitory effect of 125I-UdR on endostatin gene in rat model of cancer. Methods: Animal models of subcutaneously implanted carcinoma of Walker-256 cells were established and divided into 1-4 groups (control group, 125I-UdR group, Endostatin group and Endostatin + 125I-UdR group) The volume of saline (125I-UdR, endostatin gene, 125I-UdR and endostatin gene mixture) was injected intratumorally, and the volume of tumor before treatment (V0) and the volume after treatment 10d, 20d tumor growth rate (f = Vt / V0), observed in each group of tumor changes in light microscopy. Results: The tumor growth rates of rats in each group were (11.03 ± 1.08,27.35 ± 1.08), (4.02 ± 0.79, 7.58 ± 2.98), (3.88 ± 0.26, 7.02 ± 2.75), (2.72 ± 1.01, 2.94 ± 1.26). The tumor growth rates in groups 2, 3, and 4 were significantly less than those in group 1 (P <0.001). The differences in tumor growth rates between groups 2 and 3 were not significant (P> 0.05) 3 groups (P <0.01). CONCLUSIONS: Tumor growth can be significantly inhibited by the combination of 125I-UdR, endostatin gene and a mixture of both, by a solid intratumoral injection. The combination of endostatin gene and 125I-UdR is more effective in inhibiting tumor growth .