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目的探析核苷酸还原酶M1(ribonucleotide reductase M1,RRM1)和剪切修复偶联因子1(excision repair cross-complementing 1,ERCC1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的临床意义。方法选取自2013年8月20日—2014年10月20日来院接受治疗的43例非小细胞肺癌(non-small cell lung cancer,NSCLC)患者,应用RT-PCR方法检测RRM1和的基本表达情况。比较ERCC1与RRM1在癌组织与癌旁组织中的阳性表达情况及其在癌组织低、中、高分化等中的阳性表达率,并分析该两者间的相关性。结果 Rt-PCR(Reverse transcription-PCR)检测结果显示,非小细胞肺癌细胞中ERCC1(81.40%)与RRM1(69.76%)的阳性表达率明显高于癌旁组织(39.53%,25.58%),P<0.05。35例NSCLC患者的ERCC1表达呈阳性,其中癌组织高分化的患者比中、低分化者的阳性表达率高(P<0.05),但其在鳞癌、大细胞未分化癌和腺癌中的阳性表达差异无统计学意义(P>0.05);而30例NSCLC患者的RRM1表达呈阳性,其中癌组织低分化的患者比高、中分化者的阳性表达率高(P<0.05),在鳞癌、大细胞未分化癌和腺癌中的阳性表达中差异无统计学意义(P>0.05);且ERCC1阳性表达与RRM1阳性表达呈正相关性(相关系数r=0.841,P<0.05)。结论 ERCC1和RRM1在癌组织中表达较为活跃,且ERCC1与RRM1之间呈正相关关系并且不同的分化程度的癌组织对其阳性表达率不同,而不同癌变类型与两者的阳性表达无关。
Objective To investigate the expression of ribonucleotide reductase M1 (RRM1) and excision repair cross-complementing 1 (ERCC1) in non-small cell lung cancer (NSCLC) Clinical significance. Methods Forty-three patients with non-small cell lung cancer (NSCLC) admitted to our hospital from August 20, 2013 to October 20, 2014 were enrolled. RT-PCR was used to detect the basic expression of RRM1 Happening. The positive expression rates of ERCC1 and RRM1 in cancer tissue and paracancer tissues and their positive rates in low, moderate and high differentiated cancer tissues were compared, and the correlation between them was analyzed. Results The positive rate of ERCC1 (81.40%) and RRM1 (69.76%) in non-small cell lung cancer was significantly higher than that in adjacent non-small cell lung cancer (39.53%, 25.58%) by RTPCR <0.05.35 The expression of ERCC1 in NSCLC patients was positive. The positive expression rate of ERCC1 in patients with well-differentiated cancer tissues was higher than that in moderately-poorly differentiated patients (P <0.05), but in squamous cell carcinoma, large cell undifferentiated carcinoma and adenocarcinoma (P> 0.05). However, RRM1 expression was positive in 30 patients with NSCLC, and the positive rate of RRM1 was higher in patients with poorly differentiated cancer than those in patients with high or moderate differentiation (P <0.05) , But no significant difference was found between squamous cell carcinoma, large cell undifferentiated carcinoma and adenocarcinoma (P> 0.05). The positive expression of ERCC1 was positively correlated with the positive expression of RRM1 (r = 0.841, P <0.05) ). Conclusion The expression of ERCC1 and RRM1 in cancer tissues is relatively active. There is a positive correlation between ERCC1 and RRM1, and the positive expression rates of ERCC1 and RRM1 in different differentiation degrees are different. However, the types of cancerous lesions are not correlated with the positive expression of ERCC1 and RRM1.