目的建立羊水载玻片原位培养技术的规范化流程,并实现临床转化。方法取10例羊水标本,采用与进口载玻片培养瓶同步培养,评价该载玻片贴壁性能。取100例羊水,探索核型处理技术中低渗、预固定、染色体分散动力学等条件,建立规范化流程。另随机选择100例羊水,该载玻片与常规塑料瓶同步培养羊水细胞,评价临床应用可行性。结果配对t检验分析不同载玻片种类的细胞克隆数和优质分裂相数,差异无统计学意义(P>0.05),且该载玻片价格低廉。核型处理分析技术中显示采用1%枸橼酸钠低渗液、5%冰乙酸预固定液时,每张载玻片所获得的优质分裂相较多;选择较适分散环境温度(Temperature,T)、相对湿度(Humidity,H)组合(T=21℃,H=30%;T=21℃,H=33%),可有>60%的优质分裂相用于染色体核型分析。临床应用比对试验结果显示,两者培养成功率均为100%,配对t检验分析两种方法的细胞克隆数和优质分裂相数,差异均无统计学意义(P>0.05),核型符合率达100%,检出正常核型95例,异常核型5例,染色体多态性改变3例,18三体1例,mos 47,XX,+7[3]/46,XX[17]1例。结论该载玻片对羊水细胞的贴壁能力不亚于进口载玻片,且价格低廉,建立载玻片原位培养羊水细胞及核型处理分析技术规范化流程,初步临床应用显示与常规塑料瓶原位培养法获得同样效果,为高通量自动扫描捕获体系提供了基础技术平台,值得临床推广。 Objective To establish a standardized procedure for the in situ culture of amniotic fluid slide and achieve clinical transformation. Methods Ten cases of amniotic fluid samples were collected and cultured in the same manner as imported slide culture flasks to evaluate the adhesion properties of the slide. Take 100 cases of amniotic fluid to explore the karyotype treatment technology in the hypotonic, pre-fixed, chromosomal dispersion kinetics and other conditions, the establishment of standardized processes. Another 100 cases of amniotic fluid were randomly selected, the slide and conventional plastic bottles of amniotic fluid cells simultaneously cultured to evaluate the feasibility of clinical application. Results Paired t test was used to analyze the number of cell clones and the number of high-quality fission phases in different types of slides, with no significant difference (P> 0.05). The slides were inexpensive. Karyotyping analysis showed that 1% sodium citrate hypotonic solution and 5% glacial acetic acid pre-fixative solution were used to obtain high-quality schizonts per slide. Choosing a more appropriate ambient temperature (Temperature, T> 21 ° C, H = 30%; T = 21 ° C, H = 33%) and> 60% of high quality schizonts could be used for karyotype analysis. The results of clinical application comparison showed that the success rate of both culture was 100%. There was no significant difference between the two methods in paired t test (P> 0.05) and karyotype The results showed that there were 95 cases of normal karyotype, 5 cases of abnormal karyotype, 3 cases of chromosome polymorphism, 1 case of 18 trisomy, mos 47, XX, +7 [3] / 46, XX [17] 1 case. Conclusion The slides have the ability of adhering to amniotic fluid cells as much as the imported slides, and the price is low. The establishment of slide cells in situ culture of amniotic fluid cells and analysis of karyotyping process standardization process, preliminary clinical applications and conventional plastic bottles In situ culture method to obtain the same effect, high-throughput automated scanning capture system provides the basic technology platform, it is worth clinical promotion.