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内分泌-旁分泌细胞(APUD或神经内分泌细胞)已有报道存在于哺乳类的泌尿生殖系中,最常见的部位为前列腺和尿道。在本研究中,我们首次在豚鼠精囊腺上皮中显示出了少量的内分泌-旁分泌细胞。但由于这种细胞在精囊腺中数量极少,在切片中原位寻找甚为困难,因此我们采用酶分离细胞技术,将精囊腺上皮分离下来,离心收集制成涂片,经甲醛诱发荧光法和银染法得以证实此种细胞的存在。精囊腺从年轻成鼠体内摘除后,将粘膜面翻转向外,套在玻棒上。玻棒的另一端与一小型电动机相连,将套有精囊腺的一端插入含2%蛋白酶和0.05%EDTA的EBM(Eagle′basal mcdium)液中,在37℃中离心30～50分钟(200 rpm)。收集分离的上皮细
Endocrine-paracrine cells (APUD or neuroendocrine cells) have been reported to exist in the urogenital tract of mammals, the most common sites being the prostate and urethra. For the first time in this study, we showed a small amount of endocrine-paracrine cells in the guinea pig seminal vesicle epithelium. However, due to the very small number of such cells in the seminal vesicle, which is very difficult to find in situ in the sections, we isolated the seminal vesicle epithelial cells by enzymatic dissociation of cells, collected by centrifugation and made into smear, and formaldehyde-induced fluorescence and Silver staining confirmed the existence of such cells. After the seminal vesicle is removed from the young adult rat, the mucosal surface is flipped outwards and set over the glass rod. The other end of the glass rod was connected to a small electric motor. One end of the seminal vesicle gland was inserted into Eagle’s basal fluid (EBM) solution containing 2% protease and 0.05% EDTA and centrifuged at 37 ° C for 30 to 50 minutes (200 rpm ). Collect isolated epithelium fine