Neurturin (NTN)是对多巴胺能神经元有营养、支持和保护作用的神经营养因子 ,是帕金森病治疗的潜在有效因子。本研究用RT PCR方法获取prepro neurturincDNA ,并克隆至pBluescriptⅡSK(+)载体中 ,经酶切及测序鉴定正确 ;再将prepro neurturin基因克隆至 pcDNA3真核表达载体中 ,经Lipofectamine转染 ,在COS 7细胞中瞬间表达 ,经NorthernBlot和WesternBlot印迹分析 ,鉴定其mRNA和蛋白表达正确。用表达NTN的条件培养基处理原代培养胚胎中脑神经元 ,发现其有促进神经元存活和发育功能。 Neurturin (NTN), a neurotrophic factor that has nutritional, supportive and protective effects on dopaminergic neurons, is a potentially potent factor in the treatment of Parkinson’s disease. In this study, prepro neurturincDNA was obtained by RT-PCR and cloned into pBluescriptⅡSK (+) vector. The recombinant plasmid was identified by restriction enzyme digestion and sequencing. The prepro neurturin gene was cloned into pcDNA3 eukaryotic expression vector, transfected by lipofectamine, Cells transiently expressed by NorthernBlot and WesternBlot blot analysis to identify the correct expression of mRNA and protein. Treatment of primary cultured embryonic midbrain neurons with conditioned medium expressing NTN was found to promote neuronal survival and developmental functions.