Objective:To achieve the co-expression of GrB and PFP in Hep-2 cells and analyze the growth inhibiting effects on Hep-2 cells.Methods:Lymphocytes were separated from human laryngeal carcinoma tissue,complete Exon fragments of GrB and PFP were amplified by RT-PCR via extracting lymphocytes total RNA.and they were recombined to the downstream Of T7 promoter in the vector pVAX1.The recombinant plasmid pVAXl-PIG was transfected into Hop-2 cells with Lipofectamine 2000.The expression of proteins was identified by RT-PCR.MTT and western blot assay.Results:The gene sequence of the RT-PCR products of GrB and PFP were consistent with the data of GenBank by DNA sequencing analysis.The GrB and PFP eDNA fragment were cloned into the vector of pVAX 1 in the right direction and the open reading fragment of GrB and PFP were maintained.The target proteins were detected in the transfected Hep-2 cells.and the inhibitive effect of PFP and GrB on Hop-2 cells growth were studied by thiazolyl blue(MTT)test.Conclusion:The pVAX 1-PFP-IRES-GrB plasmid was successfully constructed and expressed,and the expression of PFP and GrB could inhibit the growth of Hep-2 cells.