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目的:探讨小儿幽门螺杆菌(Hp)感染与胃黏膜及血清白细胞介素-8(IL-8)含量的关系。方法:对43例接受胃镜检查的患儿用双抗体夹心酶联免疫吸附法测定其胃黏膜培养上清及血清中IL-8的含量,并比较Hp感染与非Hp感染患儿胃黏膜及血清中IL-8含量的差异。结果:Hp感染患儿胃黏膜中IL-8含量显著高于非Hp感染患儿(P0.05)。Hp感染组根治Hp治疗前后血清IL-8含量亦无显著变化(P>0.05)。结论:Hp感染可以诱导胃黏膜炎症细胞合成IL-8,IL-8在Hp相关性胃十二指肠疾病的发病机制中起着重要作用。
Objective: To investigate the relationship between Helicobacter pylori (Hp) infection and gastric mucosa and serum interleukin-8 (IL-8) in children. Methods: The content of IL-8 in the supernatant and serum of gastric mucosa of 43 children undergoing endoscopy was measured by double antibody sandwich enzyme-linked immunosorbent assay. The gastric mucosa and serum of children with Hp infection and non-Hp infection were compared IL-8 content in the difference. Results: The content of IL-8 in gastric mucosa of children with Hp infection was significantly higher than that of children without Hp infection (P <0.001). After Hp treatment, the content of IL-8 in gastric mucosa of children with Hp infection was significantly decreased (P <0.001), while the serum IL-8 level was not significantly different between Hp infection group and non-Hp infection group (P> 0.05). Hp infection group before and after treatment of Hp serum IL-8 content also did not change significantly (P> 0.05). Conclusion: Hp infection induces the synthesis of IL-8 by inflammatory cells in gastric mucosa. IL-8 plays an important role in the pathogenesis of Hp-related gastroduodenal diseases.