论文部分内容阅读
为测定血浆中卡托普利及其二硫键代谢物总浓度，以适应临床进行血药浓度监测。用高效液相色谱方法。样品中卡托普利二聚体及卡托普利与氨基酸、血浆蛋白的二硫键结合物采用ＮａＢＨ４还原，释放出卡托普利原形，经液—液提取纯化后，以邻苯二甲醛及Ｄ苯丙氨酸进行衍生化。选用反相ＨＰＬＣ法，荧光检测。此法线性范围为５～３００ｎｇ·ｍｌ－１，最低检测限为５ｎｇ·ｍｌ－１。用本法测定了多名高血压病患者血浆中卡托普利及其二硫键代谢物的总含量，结果证明此法灵敏度高
In order to determine the plasma total concentration of captopril and its disulfide metabolites, to adapt to the clinical monitoring of plasma concentrations. Using high performance liquid chromatography method. Samples of captopril dimer and captopril with amino acids, plasma protein disulfide bond using NaBH4 reduction, the release of captopril prototype, after liquid-liquid extraction and purification of phthalaldehyde And D phenylalanine for derivatization. Use reversed-phase HPLC method, fluorescence detection. The linear range of this method is 5 ~ 300ng · ml-1, the lowest detection limit is 5ng · ml-1. Using this method to determine the plasma total captopril and its disulfide metabolites in patients with hypertension, the results show that this method is highly sensitive