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目的探讨慢性阻塞性肺疾病(COPD)患者血清巨噬细胞移动抑制因子(MIF)的表达。方法 COPD患者100例均分为急性加重(A)组和稳定(B)组,同期体检的健康人50例予以对照(C组)。检测并比较三组患者的血清NF-κB、MIF、IL-6和C反应蛋白(CRP)的表达。结果三组血清NF-κB均无统计学差异(P>0.05);三组血浆MIF水平差异无统计学意义(P>0.05)。A组MIF mRNA高于B、C组(0.0314±0.0210vs.0.0130±0.0193、0.0175±0.0063)(P<0.05)。A组巨噬细胞CD4~+MIF~+T细胞和CD8~+MIF~+T细胞占总CD4~+T细胞的百分比均高于B、C组[(77.78±15.75)%vs.(60.39±20.71)%、(62.08±19.65)%和(76.97±19.89)%vs.(59.36±26.18)%、(55.72±28.94)%](P<0.05)。B、A组血清IL-6表达高于C组[(45.46±17.04)ng/L、(31.78±19.65)ng/L vs.(24.82±8.97)ng/L](P<0.05)。A组CRP高于B、C组[(28.23±37.56)mg/L vs.(8.23±17.21)mg/L、(2.82±4.79)mg/L](P<0.05)。结论血清巨噬细胞MIF和IL-6可能与COPD发病有关。
Objective To investigate the expression of serum macrophage migration inhibitory factor (MIF) in patients with chronic obstructive pulmonary disease (COPD). Methods One hundred patients with COPD were divided into acute exacerbation (A) group and stable (B) group, and 50 healthy subjects were enrolled in the same period (C group). The serum levels of NF-κB, MIF, IL-6 and C-reactive protein (CRP) were detected and compared among three groups. Results There was no significant difference in serum NF-κB between the three groups (P> 0.05). There was no significant difference in plasma MIF levels between the three groups (P> 0.05). The MIF mRNA of group A was higher than that of group B and C (0.0314 ± 0.0210 vs.0.0130 ± 0.0193 and 0.0175 ± 0.0063 respectively) (P <0.05). The percentages of CD4 ~ + MIF ~ + T cells and CD8 ~ + MIF ~ + T cells in group A were higher than that in group B and C [(77.78 ± 15.75)% vs (60.39 ± (62.08 ± 19.65)% and (76.97 ± 19.89)% vs (59.36 ± 26.18)%, (55.72 ± 28.94)%] (P <0.05). The serum levels of IL-6 in group B and group A were significantly higher than those in group C [(45.46 ± 17.04) ng / L, (31.78 ± 19.65) ng / L vs. (24.82 ± 8.97) ng / L], respectively. The CRP in group A was higher than that in group B and C [(28.23 ± 37.56) mg / L vs. (8.23 ± 17.21) mg / L, (2.82 ± 4.79) mg / L], respectively. Conclusion Serum macrophages MIF and IL-6 may be related to the pathogenesis of COPD.