Determination of IC_(50) for Nine Medicinal Plants against α-Glucosidase Activity

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[Objective] To screen the plant-derived α-glucosidase inhibitors. [Method] Using the half maximal inhibitory concentration (IC50) as the potency index, the inhibitory effects of water extracts from 34 kinds of traditional Chinese medicines on α-glucosidase activity were evaluated. [Result] When they were used in concentrations of 50 mg/ml, the water extracts from RADIX MORINDAE OFFICINALIS, RADIX ACHYRANTHIS BIDENTATAE, RHIZOMA POLYGONATI, RADIX PAEONIAE ALBA, RADIX POLYGONI MULTIFLORI, FRUCTUS SCHISANDRAE CHINENSIS, RHIZOMA CHUANXIONG, RHIZOMA ET RADIX POLYGONI CUSPIDATI, RHIZOMA DIOSCOREAE NIPPONICAE showed inhibitory effects on α-glucosidase activity with inhibition rate over 50%. Further researches found that the inhibition rate of water extracts from FRUCTUS SCHISANDRAE CHINENSIS, RHIZOMA CHUANXIONG and RHIZOMA ET RADIX POLYGONI CUSPIDATI on α-glucosidase activity was all higher than 80%. The above 3 kinds of traditional Chinese medicines were ranked in descending order of inhibitory effects: RHIZOMA ET RADIX POLYGONI CUSPIDATI > FRUCTUS SCHISANDRAE CHINENSIS > RHIZOMA CHUANXIONG. Among them, there were 3 medicinal plants with IC50 below 40, which were arranged in order of efficiency strength, RADIX POLYGONI MULTIFLORI > RADIX PAEONIAE ALBA > RHIZOMA ET RADIX POLYGONI CUSPIDATI. [Conclusion] It is feasible to screen hypoglycemic drugs from plant resources. This study can provide reference for discovering high-efficiency and safe biological drugs of reducing postprandial blood glucose. [Objective] To screen the plant-derived α-glucosidase inhibitors. [Method] Using the half maximal inhibitory concentration (IC50) as the potency index, the inhibitory effects of water extracts from 34 kinds of traditional Chinese medicines on α-glucosidase activity were The results were shown in Table 1. [Result] When they were used in concentrations of 50 mg / ml, the water extracts from RADIX MORINDAE OFFICINALIS, RADIX ACHYRANTHIS BIDENTATAE, RHIZOMA POLYGONATI, RADIX PAEONIAE ALBA, RADIX POLYGONI MULTIFLORI, FRUCTUS SCHISANDRAE CHINENSIS, RHIZOMA CHUANXIONG, RHIZOMA ET RADIX POLYGONI CUSPIDATI, RHIZOMA DIOSCOREAE NIPPONICAE showed inhibitory effects on α-glucosidase activity with inhibition rate over 50%. Further researches found that the inhibition rate of water extracts from FRUCTUS SCHISANDRAE CHINENSIS, RHIZOMA CHUANXIONG and RHIZOMA ET RADIX POLYGONI CUSPIDATI on α-glucosidase activity was all higher than 80%. The above 3 kinds of traditional Chinese medicines were ranked in descending order of inh ibitory effects: RHIZOMA ET RADIX POLYGONI CUSPIDATI> FRUCTUS SCHISANDRAE CHINENSIS> RHIZOMA CHUANXIONG. Among them, there were 3 medicinal plants with IC50 below 40, which were arranged in order of efficiency strength, RADIX POLYGONI MULTIFLORI> RADIX PAEONIAE ALBA> RHIZOMA ET RADIX POLYGONI CUSPIDATI. [Conclusion] It is feasible to screen for hypoglycemic drugs from plant resources. This study can provide reference for discovering high-efficiency and safe biological drugs of reducing postprandial blood glucose.
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