目的 :观察射线对神经元存活能力的影响。方法 :应用大鼠 E1 8脊髓细胞 ,在培养第 2天进行 4Gy X线定量照射。结果 :培养 1、2、3、4周时 ,神经元分别占生存细胞的 89.31%、96 .78%、80 .42 %、46 .42 % ,明显高于对照组。结论 :该方法能获得较大数量的存活神经元细胞 ,可以增加对神经元研究观察的时间跨度 ,明显优越于细胞毒性药物加入培养基中的方法 Objective: To observe the effects of radiation on the survival of neurons. METHODS: Rat E1 8 spinal cord cells were used for quantitative 4Gy X-ray irradiation on day 2 of culture. Results: Neurons accounted for 89.31%, 96.78%, 80.42% and 46.42% of the surviving cells at 1, 2, 3 and 4 weeks respectively, which were significantly higher than that of the control group. Conclusion: This method can obtain a larger number of surviving neuronal cells, which can increase the time span of observation of neurons and is obviously superior to the method of adding cytotoxic drugs to the culture medium