目的探讨七叶皂苷钠对油酸诱导大鼠急性肺损伤模型的干预作用。方法♂SD大鼠54只随机分为5组,分别为正常对照组、注射七叶(非油酸造模)组、油酸造模组、甲基强的松龙组和七叶皂苷钠组。采用鼠尾静脉缓慢注射油酸(OA,0.1ml.kg-1)复制大鼠急性肺损伤模型,模型复制成功后按相应措施处理观察并取血取材检测相关指标。观察检测指标有肺组织形态学、肺湿/干重(W/D)、光镜下半定量肺损伤评分(IQA)、血气分析动脉血氧分压(PaO2)、血浆及肺组织匀浆超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果①肺组织形态学:七叶皂苷钠组及甲基强的松龙组肺表面充血程度较油酸组明显减轻,气管内粉红色分泌物溢出减少;光镜下,七叶皂苷钠组及甲基强的松龙组较油酸造模组肺泡腔内炎症细胞渗出减轻;②肺湿/干重(W/D):油酸造模组W/D较空白对照组明显升高,七叶皂苷钠组及甲基强的松龙组W/D较油酸造模组明显降低;③光镜下半定量肺损伤评分(IQA):油酸造模组IQA较空白对照组明显升高,七叶皂苷钠组及甲基强的松龙组IQA较油酸造模组明显降低;④血气分析动脉血氧分压(PaO2):油酸造模组PaO2较空白对照组明显降低,七叶皂苷钠组及甲基强的松龙组PaO2较油酸造模组升高;⑤血浆及肺组织匀浆超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量:油酸造模组血浆及肺组织SOD活性较空白对照组明显降低,七叶皂苷钠组及甲基强的松龙组血浆和肺组织SOD活性较油酸造模组明显升高;油酸造模组血浆及肺组织MDA含量较空白对照组明显升高,七叶皂苷钠组及甲基强的松龙组血浆和肺组织MDA含量较油酸造模组明显降低。结论七叶皂苷钠通过对急性肺损伤大鼠氧化应急的调节作用,以改善W/D、IQA、PaO2,从而为临床治疗急性肺损伤提供一个可能的途径。 Objective To investigate the effects of sodium aescinate on oleic acid-induced acute lung injury in rats. Methods Fifty-four male SD rats were randomly divided into 5 groups: normal control group, injection of Aesculi (non-oleic acid) model group, oleic acid model group, methylprednisolone group and sodium aescinate group . Rat model of acute lung injury was induced by slow injection of oleic acid (OA, 0.1ml.kg-1) into rat caudal vein. After successful replication of the model, the corresponding measures were taken according to the corresponding measures. Lung function, lung wet / dry weight (W / D), semi-quantitative lung injury score (IQA), blood gas analysis PaO2, plasma and lung homogenate SOD activity and malondialdehyde (MDA) content. Results ① The morphological changes of lung: the hyperemia of lung surface of sodium aescinate group and methylprednisolone group was significantly reduced than that of oleic acid group, and the overflow of pink secretions in the trachea decreased. Under the light microscope, Methylprednisolone group than oleic acid group alveolar intraluminal inflammatory cell exudate reduced; wet lung / dry weight (W / D): oleic acid model group W / D was significantly higher than the control group, Compared with oleic acid group, the W / D of sodium aescinate group and methylprednisolone group was significantly lower than that of oleic acid group; ③ IQA of light-microscope group: IQA of oleic acid group was significantly higher than that of blank control group High, aescin sodium group and methylprednisolone group IQA significantly lower than the oleic acid model group; ④ blood gas analysis of arterial oxygen pressure (PaO2): oleic acid model PaO2 control group was significantly lower than the control group, Sodium aescinate and methylprednisolone group PaO oleic acid model group increased; ⑤ plasma and lung homogenate superoxide dismutase (SOD) activity and malondialdehyde (MDA) content: oleic acid SOD activity in plasma and lung tissue in model group was significantly lower than that in blank control group. SOD activity in plasma and lung tissue of sodium aescinate group and methylprednisolone group was significantly higher than that in oleic acid group; The levels of MDA in plasma and lung tissue in model group were significantly higher than those in blank control group. The contents of MDA in plasma and lung tissue in sodium aescinate group and methylprednisolone group were significantly lower than those in oleic acid group. Conclusion Sodium aescinate can improve the W / D, IQA and PaO2 by regulating the oxidative stress in rats with acute lung injury and provide a possible approach for the treatment of acute lung injury.