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目的:建立一种RP-HPLC方法,用于分离测定甘葛藤茎、叶中葛根素、大豆苷和大豆苷元的含量,为甘葛藤茎、叶的开发利用提供依据。方法:采用C18色谱柱,甲醇(A)-1%冰醋酸(B)溶液为流动相,梯度洗脱,流速为1 mL.min-1,检测波长250 nm,柱温25℃。结果:3个化合物在测定的范围内具有良好的线性(r>0.9995),方法的回收率在99.0%~101.6%。甘葛藤的茎中葛根素、大豆苷和大豆苷元的含量较叶中高。结论:所建立的方法准确、可靠,可作为甘葛藤的茎、叶中葛根素、大豆苷和大豆苷元含量测定的方法。
Objective: To establish a RP-HPLC method for the separation and determination of puerarin, daidzein and daidzein in the stems and leaves of Candida glabra. This will provide a basis for the development and utilization of the stems and leaves of the cane. METHODS: A C18 column was used. The methanol (A)-1% glacial acetic acid (B) solution was used as the mobile phase. The gradient elution was performed at a flow rate of 1 mL.min-1. The detection wavelength was 250 nm and the column temperature was 25°C. RESULTS: The three compounds had good linearity (r>0.9995) within the range of determination. The recoveries of the method ranged from 99.0% to 101.6%. The contents of puerarin, daidzin, and daidzein in the stems of the cane were higher than those in the leaves. Conclusion: The established method is accurate and reliable. It can be used as a method for the determination of puerarin, daidzein and daidzein in stems and leaves of G. straminea.