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目的:测量与分析肝细胞癌DNA干系倍体及其临床意义。方法:使用TIGER细胞图像分析仪测量 4 5例肝细胞癌组织 4 μm、10 μm切片上DNA干系倍体值。4 μm组织切片测量肝细胞癌细胞核DNA的光密度,10 μm组织切片测量单个完整肝细胞癌细胞核的体积,经TIGER细胞图像分析仪计算获得以单个完整肝细胞癌细胞核体积为单位的DNA总量(以体积积分光密度表述),以同一切片内正常淋巴细胞作为内对照,计算其DNA干系倍体值。结果:⑴无 1例DNA干系倍体为二倍体;DNA干系倍体值在 2～ 5范围者 11例;在 5～ 8范围者 2 8例;大于 8者 6例。⑵DNA干系倍体与瘤体大小、有无淋巴结转移、组织学分级、术后生存率等有相关性。结论:DNA干系倍体能较准确反映肝细胞癌的生物学特性,为认识肝细胞癌的病理学特征以及判断预后提供了有价值的客观依据。
Objective: To measure and analyze hepatocellular carcinoma DNA-dried ploidy and its clinical significance. Methods: TGA cell image analyzer was used to measure the DNA ploidy in 4 and 10 μm sections of 45 hepatocellular carcinoma tissues. 4 μm tissue sections were used to measure the optical density of nuclear DNA of hepatocellular carcinoma cells. The volume of nuclei of single intact hepatocellular carcinoma cells was measured by 10 μm tissue sections. The total amount of DNA in single intact hepatocellular carcinoma cell nuclei was calculated by TIGER image analyzer (Expressed in terms of volume integral optical density), normal lymphocytes in the same slice as an internal control, calculate the DNA dry ploidy value. Results: (1) None of the 1 cases had diploid diploid of DNA dry line; 11 cases of DNA dry ploidy in 2 ~ 5 range; 28 cases in range of 5 ~ 8; 6 cases of more than 8 cases. ⑵ DNA dry line ploidy and tumor size, with or without lymph node metastasis, histological grade, postoperative survival rate and other related. CONCLUSION: DNA-based haploid multiples reflect the biological characteristics of hepatocellular carcinoma more accurately and provide valuable objective evidences for understanding the pathological features and prognosis of hepatocellular carcinoma.