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目的:探讨血管钠肽(Vasonatrin peptide,VNP)对碾压撕脱伤皮瓣P-选择素表达的影响。方法:24只大鼠随机分为3组(n=8),分别为SHAM手术组、碾压撕脱组及VNP+碾压撕脱组,于术前、术后12h及24取材,用免疫组织化学染色标记不同方式处理组皮瓣中P-选择素的表达。结果:碾压撕脱组皮瓣中P-选择素表达水平较高,而采用VNP治疗组皮瓣中P-选择素表达量较前者显著降低(P<0.05)。结论:VNP可显著降低碾压撕脱皮瓣中P-选择素的表达量,进而减轻炎症反应并抑制血栓形成,可为临床上提高碾压撕脱皮瓣成活率提供一种新的治疗思路。
Objective: To investigate the effect of Vasonatrin peptide (VNP) on the expression of P-selectin in skin flap of crushed avulsion injury. Methods: Twenty-four rats were randomly divided into 3 groups (n = 8): SHAM operation group, rolling avulsion group and VNP + rolling avulsion group. Chemical staining marked different ways to treat P-selectin expression in group flap. Results: The expression of P-selectin was higher in the skin flap of roller compacted avulsion group, while the expression of P-selectin in the flap of VNP group was significantly lower than the former (P <0.05). Conclusion: VNP can significantly reduce the expression of P-selectin in the skin flap after rolling, thereby reducing the inflammatory reaction and inhibiting the thrombosis, which may provide a new therapeutic approach for improving the survival rate of the roller-bed avulsion flap .