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目的 :检测角膜移植排斥反应房水中肿瘤坏死因子 α(tumornecrosisfactoralpha ,TNF α)含量及其与角膜移植免收稿日期 :2 0 0 2 -11-19;修回日期 :2 0 0 3 -0 1-2 2作者简介 :李贵仁 (1943 -) ,男 ,山东潍坊人 ,主任医师 ,研究方向 :角膜病。通信作者 :李贵仁 (E -mail:li-yan2 0 0 2 0 0 1@163 .com)疫排斥反应的关系。方法 :将健康新西兰白兔 4 4只随机分为 4组。A组 :正常对照组 8只 ;B组 :自体穿透性角膜移植组 12只 ;C组 :异种异体穿透性角膜移植后用CsA治疗组 12只 ;D组 :异种异体穿透性角膜移植组 12只。供体为新鲜鸡角膜。分别于术后第 3、第 7、第 14、第 2 1、第 2 8天采取兔耳缘动脉血和房水 ,应用酶联免疫吸附 (ELISA)双抗体夹心法检测房水及外周血中TNF α的含量 ,并对房水细胞行免疫组化染色观察。结果 :正常血清和房水中均可检测到少量的TNF α ,它们的浓度分别为 (6 9.98± 17.4 0 )pg/ml和 (31.2 5±17.13)pg/ml。术后早期D组房水及外周血中TNF α含量即升高 ,至排斥反应前达最高水平 ,在观察期 (2 8d)内维持高水平。血、房水TNF α含量变化呈密切正相关 (r =0 .94 ,P <0 .0 1)。排斥反应发生时 ,D组房水涂片、瑞氏染色可见较多淋巴细胞和巨噬细胞 ,而相同时间的A、B组房水均未发现?
OBJECTIVE: To detect the content of tumor necrosis factor α (TNF α) in corneal allograft rejection water and its effect on the corneal transplantation free of charge. Received: 2002-02-11; Revised: 2 0 0 3 -0 1 -2 2 About the author: Li Guiren (1943 -), male, Shandong Weifang people, chief physician, research direction: corneal disease. Corresponding author: Li Guiren (E-mail: li-yan2 0 0 1 1 163 .com) The relationship between the rejection. Methods: Forty four healthy New Zealand white rabbits were randomly divided into four groups. Group A: normal control group of 8; Group B: autologous penetrating keratoplasty group of 12; Group C: xenograft penetrating keratoplasty after CsA treatment group of 12; Group D: xenograft penetrating keratoplasty Group of 12. Donor for fresh chicken cornea. Rabbit ear arteries and aqueous humor were collected on the 3rd, 7th, 14th, 21st, 28th days after operation respectively. Atrial fibrillation and aqueous humor were detected by enzyme-linked immunosorbent assay (ELISA) TNFα content, and the aqueous humor cells line immunohistochemical staining. Results: A small amount of TNFα was detected in both normal serum and aqueous humor. Their concentrations were (6 9.98 ± 17.4 0) pg / ml and (31.2 5 ± 17.13) pg / ml, respectively. In the early postoperative period, the level of TNFα in aqueous humor and peripheral blood of group D increased to the highest level before rejection and maintained high level during the observation period (28 days). Blood and aqueous TNFα levels were closely related (r = 0.94, P <0.01). Exclusion reaction occurred, D group aqueous humor, Wright staining more lymphocytes and macrophages, and the same time, A, B group were not found in aqueous humor?