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为探讨p1 6基因甲基化与儿童急性白血病 (AL)的发生、发展、预后的关系 ,采用酶切加PCR技术研究 33例初治儿童AL ,1 5例复发儿童AL及 30例对照组p1 6基因甲基化情况 ,并观察p1 6基因甲基化与化疗结果的关系。结果 33例初治AL有 3例、1 5例复发AL有 6例、30例对照组中 0例存在p1 6基因甲基化。可评价疗效的 31例初治AL中 ,3例甲基化患者未缓解 ;2 8例非甲基化者中 3例获完全缓解 ,2 3例部分缓解 ,2例未缓解 (总有效率 92 .86 % )。结果表明 :(1 )在初治儿童AL中p1 6基因甲基化率低 ,可能不是儿童AL的主要发病机制 ;(2 )p1 6基因甲基化率儿童AL复发病例显著上升 ,值得重视 ,同时对指导治疗及预测病程和预后具有重要意义。
In order to investigate the relationship between the methylation of p16 gene and the occurrence, development and prognosis of childhood acute leukemia (AL), 33 children with AL, 15 with recurrent AL and 30 with control group p1 6 gene methylation and observe the relationship between p16 methylation and chemotherapy results. Results 33 cases of initial treatment of AL in 3 cases, 15 cases of recurrent AL in 6 cases, 30 cases of control group 0 cases of p1 6 gene methylation. Among the 31 patients with initial AL that could evaluate the efficacy, 3 patients with methylation did not relieve; 3 of 28 patients with unmethylation were completely relieved, 23 patients were partially relieved, 2 patients did not relieve (the total effective rate was 92 .86%). The results showed that: (1) The low methylation rate of p16 gene in AL children may not be the main pathogenesis of AL in children with AL; (2) The incidence of AL recurrence in children with methylation of p16 gene is significantly increased, At the same time to guide the treatment and prediction of disease duration and prognosis of great significance.