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目的检测缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在肾细胞癌中的表达及其相互关系。方法用免疫组织化学SABC法检测42例肾细胞癌组织中HIF-1α及VEGF蛋白的表达。结果肾细胞癌组织中HIF-1α阳性表达率为57.1%,癌旁及正常组织不表达;肾细胞癌VEGF阳性表达率为61.9%,与癌旁及正常组织(阳性表达率为20%)相比有显著性差异(P<0.05);癌转移患者与无癌转移者HIF-1α、VEGF比较均有显著性差异(P<0.01);HIF-1α与VEGF表达呈正相关(Kappa=0.41,P<0.01)。结论HIF-1α在肾细胞癌组织中呈高表达,其与VEGF有相关性,有望成为判断肾细胞癌转移和预后等生物学行为的重要参考指标,HIF-1α是VEGF表达的调控因子之一。
Objective To detect the expression of HIF-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in renal cell carcinoma and their correlation. Methods Immunohistochemical SABC method was used to detect the expression of HIF-1α and VEGF in 42 cases of renal cell carcinoma. Results The positive expression rate of HIF-1α in renal cell carcinoma tissues was 57.1%, while the adjacent non-cancerous tissues and normal tissues were not expressed. The positive expression rate of VEGF in renal cell carcinoma was 61.9%, which was significantly higher than that in para-carcinoma and normal tissues (positive rate was 20%) (P <0.01). The expression of HIF-1α and VEGF was positively correlated with the expression of VEGF (Kappa = 0.41, P <0.01) ). Conclusions HIF-1α is overexpressed in renal cell carcinoma and is correlated with VEGF. HIF-1α is expected to be an important reference marker for determining the biological behaviors such as metastasis and prognosis of renal cell carcinoma. HIF-1α is one of the regulators of VEGF expression .