论文部分内容阅读
本研究以玉米细胞质不育系cms-合344为供体亲本、辐63018为轮回亲本构建BC1和BC2群体,研究利用SSR标记进行遗传背景选择加速回交转育不育系的效果。选用两亲本间多态性好的并覆盖玉米10条染色体的60个SSR标记进行轮回亲本的遗传背景分析。结果表明,对两群体遗传背景回复率分析,86株BC1群体背景回复率平均值为74.41%;63株BC2群体背景回复率平均值为87.36%;BC1群体表型与分子标记辅助选择相似度均较高的株系有9株,BC2有8株;对两群体进行不同标记数目分析,两群体中均使用40个标记与总数60个标记所计算的背景回复率基本相同,相关系数分别达到0.96和0.95以上。本结果为回交转育不育系提供了研究基础。
In this study, we constructed a BC1 and BC2 population with maize cytoplasmic male sterility line cms-He 344 as a donor parent and a cross with 63018 as a recurrent parent to study the effect of using SSR markers to accelerate genetic selection of backcross-sterile CMS lines. Sixty SSR markers with good polymorphisms and covering 10 maize chromosomes were selected for the genetic background analysis of the recurrent parents. The results showed that the average background recovery rate of 86 BC1 population was 74.41%. The mean of the background recovery rate of 63 BC2 population was 87.36%. The similarity of BC1 population phenotype and molecular marker-assisted selection There were 9 higher strains and 8 BC2 strains. The analysis of the number of different markers in both groups showed that the average background recovery rates of 40 markers and 60 markers in both groups were basically the same, with correlation coefficients of 0.96 And 0.95 and above. The results provided the basis for the research of backcrossing CMS.