论文部分内容阅读
目的探讨G蛋白偶联雌激素受体(GPER)在子宫内膜腺癌组织中发生发展的作用。方法选取2005年3月至2008年4月郑州大学第一附属医院病理科手术及活检标本的存档蜡块,采用免疫组织化学SP法检测55例子宫内膜腺癌、49例子宫内膜增殖症及10例正常增生期子宫内膜组织中GPER蛋白的表达,分析其与子宫内膜腺癌患者临床病理特征的关系。结果正常增生期子宫内膜组织、子宫内膜增殖症组织和子宫内膜腺癌组织中GPER蛋白的阳性表达率分别为20.0%、67.3%和81.8%,差异具有统计学意义(χ2=15.778,P<0.001)。子宫内膜增殖症中,简单型增生、复杂型增生及不典型增生内膜组织中GPER蛋白的阳性表达率分别为30.0%、70.0%和84.2%,差异具有统计学意义(χ2=8.864,P=0.012)。绝经后和未绝经子宫内膜腺癌组织中GPER的阳性表达率分别为89.7%和62.5%,差异具有统计学意义(χ2=3.977,P=0.046)。高分化和中、低分化子宫内膜腺癌组织中GPER的阳性表达率分别为71.4%和100%,差异具有统计学意义(χ2=5.196,P=0.023)。临床分期Ⅰ期和(Ⅱ+Ⅲ)期子宫内膜腺癌组织中GPER的阳性表达率分别为73.0%和100%,差异具有统计学意义(χ2=4.268,P=0.039)。有淋巴结转移和无淋巴结转移的子宫内膜腺癌组织中GPER的阳性表达率分别为66.7%和83.7%,差异无统计学意义(χ2=0.210,P=0.646)。在肌层浸润深度<1/2和≥1/2的子宫内膜腺癌组织中GPER的阳性表达率分别为75%和100%,差异无统计学意义(χ2=3.057,P=0.080)。结论 GPER与子宫内膜腺癌的发生、发展可能具有相关性。
Objective To investigate the role of G protein-coupled estrogen receptor (GPER) in the development of endometrial adenocarcinoma. Methods The archived paraffin blocks of the pathology and biopsy specimens of the First Affiliated Hospital of Zhengzhou University from March 2005 to April 2008 were collected. The immunohistochemical SP method was used to detect 55 cases of endometrial adenocarcinoma and 49 cases of endometrial hyperplasia And 10 cases of normal proliferative endometrial tissue GPER protein expression, and its relationship with clinicopathological features of patients with endometrial adenocarcinoma. Results The positive rates of GPER protein expression in normal proliferative endometrium, endometrial hyperplasia and endometrial adenocarcinoma were 20.0%, 67.3% and 81.8%, respectively, with statistical significance (χ2 = 15.778, P <0.001). In endometrial hyperplasia, the positive expression rate of GPER protein in simple hyperplasia, complex hyperplasia and atypical hyperplasia was 30.0%, 70.0% and 84.2%, respectively, with significant difference (χ2 = 8.864, P = 0.012). The positive rates of GPER in postmenopausal and endometrial adenocarcinoma were 89.7% and 62.5%, respectively, with statistical significance (χ2 = 3.977, P = 0.046). The positive rates of GPER in highly differentiated and moderately differentiated and poorly differentiated endometrial adenocarcinomas were 71.4% and 100%, respectively, with statistical significance (χ2 = 5.196, P = 0.023). The positive expression rates of GPER in stageⅠand (Ⅱ + Ⅲ) endometrial adenocarcinoma were 73.0% and 100% respectively, with statistical significance (χ2 = 4.268, P = 0.039). The positive rates of GPER in endometrial adenocarcinoma with lymph node metastasis and non-lymph node metastasis were 66.7% and 83.7%, respectively, with no significant difference (χ2 = 0.210, P = 0.646). The positive rate of GPER in endometrial adenocarcinoma with myometrial invasion depth <1/2 and ≥1 / 2 was 75% and 100%, respectively, with no significant difference (χ2 = 3.057, P = 0.080). Conclusion GPER may be related to the occurrence and development of endometrial adenocarcinoma.