论文部分内容阅读
目的:对比运动和雌激素对去卵巢大鼠股骨骨密度和生物力学性能的影响。方法:将40只5月龄雌性SD大鼠随机分为基础对照组(Basal)、假手术组(Sham)、去卵巢组(OVX)、去卵巢加雌激素干预组(OVX+E)、去卵巢加运动干预组(OVX+Run)。OVX+E组在手术1周后开始每天给予肌肉注射己烯雌酚(0.025mg/kg,持续12周)。OVX+Run组术后1周开始采用大鼠专用跑笼进行运动训练(跑速6.4m/min,1次/天,持续12周)。术后13周,对全部大鼠进行股骨骨密度和生物力学指标的测定。结果:(1)OVX+E组BMD值与OVX组无明显差别;OVX+Run组BMD分别较OVX组和OVX+E组显著增加(P<0.05);(2)OVX+Run组、OVX+E组极限剪切载荷、剪切强度极限、剪切弹性模量和最大剪应变显著高于OVX组(P<0.05),而与Sham组比较差别无统计学意义;OVX+Run与OVX+E组比较,剪切弹性模量明显升高(P<0.05)。结论:运动和雌激素均能显著提高去卵巢大鼠股骨骨密度和骨生物力学性能,但运动能够更好地提高骨硬度和增强骨抵抗变形的能力。
Objective: To compare the effects of exercise and estrogen on the femoral bone mineral density and biomechanical properties of ovariectomized rats. Methods: Forty five-month-old female Sprague-Dawley rats were randomly divided into basal group, Sham group, ovariectomized group, ovariectomized group and ovariectomized group (OVX + E) Ovary plus exercise intervention group (OVX + Run). OVX + E group was intramuscularly injected with diethylstilbestrol (0.025 mg / kg for 12 weeks) one week after surgery. One week after operation, the OVX + Run group was trained with a special running cage for rats (running speed 6.4m / min, once a day for 12 weeks). At 13 weeks after operation, the femur BMD and biomechanical parameters were measured in all the rats. Results: (1) There was no significant difference in BMD between OVX + E group and OVX group; BMD in OVX + Run group was significantly higher than OVX group and OVX + E group (P <0.05); (2) The ultimate shear load, shear strength limit, shear modulus and maximum shear strain in group E were significantly higher than those in OVX group (P <0.05), but no significant difference was found between Sham group and OVX + Run group Compared with the control group, the shear modulus increased significantly (P <0.05). Conclusion: Both exercise and estrogen can significantly increase femoral bone mineral density and biomechanical properties in ovariectomized rats, but exercise can improve bone stiffness and enhance the ability of bone resisting deformation.