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1982～1984年,通过对不同抗性的品种田间定穗系统观察结果表明,生育期相似的品种,抗侵入和抗扩展的能力明显不同,抗病和感病的品种,目测就能区分,因而提出用目测法淘汰大量感病品种。实践证明,应用此法可大大节省劳力,对品种资源和杂交后代的抗性鉴定,具有实用价值。小麦品种的抗扩展性鉴定,大田采用浸菌纸片、剪颖滴菌和注射针滴注,进行单花定位接种,以病害从接种点扩展为害的小穗数,把病情分为五级,取病害反应级的平均值作为评价品种抗扩展性的标准。经方差分析,品种间的F值为30.27,差异极显著,方法间的F值为0.96,差异不明显。室内单花定位接种结果同田间一致,与品种原定抗性相验证,吻合率达80％以上。四种抗性类型品种,单花定位接种后第8天,经病理切片证明,菌丝蔓延到穗轴的速度与品种的抗性有一定的关系。
From 1982 to 1984, the results of field-specific spike-fixing systems of different resistant varieties showed that the cultivars with similar growth periods had significantly different anti-invasive and anti-expansion abilities. The resistant and susceptible cultivars could be distinguished by visual inspection Proposed to use visual method to eliminate a large number of susceptible varieties. Practice has proved that the application of this method can greatly save labor, the variety of resources and cross-offspring of resistance identification, has practical value. Expansion of identification of wheat varieties, the field using dipping paper, cut the leaf cloacae and injection needle instillation, single flower positioning vaccination, the disease spread from the point of injury to the number of spikelets, the disease is divided into five levels, Take the average of the disease response level as the standard to evaluate the varieties’ scalability. The analysis of variance showed that the F value was 30.27 among varieties, the difference was extremely significant, the F value between the methods was 0.96, the difference was not obvious. Indoor single flower positioning inoculation results with the field consistent with the original proof of the species phase validation, anastomosis rate of 80% or more. Four resistant varieties, single flower positioning on the 8th day after inoculation, the biopsy showed that the mycelium spread to the cob and the resistance of varieties have a certain relationship.