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目的评价LIAISON化学发光免疫分析法(CLIA)检测人巨细胞病毒(HCMV)特异性IgM抗体的方法学特点。方法采用CLIA法对婴儿肝病综合征患者血清进行HCMVIgM检测,同时用ELISA试验进行同一批样本中部分的测定。结果CLIA检测584例,阳性132例,阳性率22.6%。两种检测方法的一致性为88.8%,但CLIA法的敏感性和特异性均高于ELISA(P<0.05)。结论CLIA检测HCMVIgM具有灵敏度高、检测所需时间短、人为影响因素少、结果准确等优点,适用于临床检测。
Objective To evaluate the methodological features of LIAISON chemiluminescence immunoassay (CLIA) for detection of human cytomegalovirus (HCMV) -specific IgM antibodies. Methods HCMVIgM was detected by CLIA in serum of infants with hepatopathy syndrome, and the ELISA assay was used to determine the partial contents of the same batch of samples. Results CLIA test 584 cases, 132 cases were positive, the positive rate of 22.6%. The consistency of the two detection methods was 88.8%, but the sensitivity and specificity of CLIA method were higher than ELISA (P <0.05). Conclusion CLIA detection of HCMVIgM has the advantages of high sensitivity, short testing time, less human factors and accurate results, which is suitable for clinical detection.