目的探讨应用Leica-CM1900型低温恒冷切片机在术中胸腹水快速制作的意义。方法取术中胸腹水底部液体直接滴加在冷冻托上速冻,与肿瘤组织冷冻切片方法相同,共同固定、HE染色。结果红蓝对比清晰,肿瘤细胞保持原有结构形态,范围局限,易于观察。结论此方法简便,容易操作,易于推广。 Objective To explore the significance of rapid production of pleural and ascitic fluid using the Leica-CM1900 cryostat microtome. Methods The intrathoracic ascites fluid was directly dripped and frozen on the frozen tray. The frozen sections were the same as those of the tumor tissue, and were fixed together and stained with HE. The results of a clear contrast of red and blue, tumor cells to maintain the original structure, scope, easy to observe. Conclusion This method is simple, easy to operate, easy to promote.