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目的:建立连钱草药材中黄酮、有机酸类成分的HPLC指纹图谱分析方法,为整体控制和评价连钱草药材的质量提供依据。方法:采用大连依利特Hypersil BDS C18(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸梯度洗脱,流速1.0mL·min-1,检测波长330 nm。结果:建立了连钱草药材黄酮、有机酸类成分的HPLC指纹图谱,标定了17个共有峰,其精密度、稳定性、重复性均符合指纹图谱技术要求(RSD<5%),13批连钱草药材的相似度在0.713～0.987。结论:该指纹图谱方法简便、准确、重复性好,可作为连钱草药材质量控制的依据。
OBJECTIVE: To establish an HPLC fingerprinting method for the determination of flavonoids and organic acids in the Chinese medicinal materials, and provide the basis for the overall control and evaluation of the quality of the medicinal materials. Methods: A HPLC gradient elution was performed on a Hypersil BDS C18 (4.6 mm × 250 mm, 5 μm) eluting with methanol-0.1% phosphoric acid at a flow rate of 1.0 mL · min-1 with a detection wavelength of 330 nm. Results: The HPLC fingerprints of flavonoids and organic acids were established and 17 common peaks were calibrated. The precision, stability and repeatability of the HPLC fingerprints were all accordant with the requirements of fingerprinting (RSD <5%), 13 batches The similarity of even medicinal herbs ranged from 0.713 to 0.987. Conclusion: The fingerprinting method is simple, accurate and reproducible, which can be used as the basis for the quality control of Chinese medicinal materials.