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本研究利用番茄黄化曲叶病毒病高感染株系7969-19-1-1-3及其高抗番茄黄化曲叶病毒病的株系Z3(Ty-3a)构建了包含1 404个单株的F2分离群体,并利用分子标记技术对其进行了分析。研究结果显示,在348对SSR、CAPS、SNP、Indel以及RFLP引物中,在双亲间有68对引物扩增出多态性差异,利用获得的68对引物对抗感池进行筛选,获得了7对多态性的标记C2_At3g56230、X38、H5、C4、TGS2216、X1和H12。为了进一步缩小区间,利用F2群体的1 404个单株进行连锁分析。在C2_At3g56230-H12区间寻找新的标记,最后将Ty-3a定位于标记TGS12660和TY3a-P19之间,2个标记的之间的物理距离约为29.7 kb,遗传距离约为0.3 c M。研究结果可为进一步克隆Ty-3a基因和分子标记辅助选择培养抗番茄黄化曲叶病毒番茄新品种奠定基础。
In this study, the tomato yellow curl leaf highly virulent strain 7969-19-1-1-3 and its high resistance to tomato yellow curl leaf virus strain Z3 (Ty-3a) constructed contains 1 404 single Strains were isolated from F2 populations and analyzed using molecular markers. The results showed that among 348 pairs of SSR, CAPS, SNP, Indel and RFLP primers, 68 pairs of primers amplified polymorphisms among parents, and 68 pairs of primers were used to screen the sense pools. Seven pairs Polymorphic markers C2_At3g56230, X38, H5, C4, TGS2216, X1 and H12. To further narrow the interval, 1 404 individuals of the F2 population were used for linkage analysis. Finally, Ty-3a was mapped between TGS12660 and TY3a-P19 in C2_At3g56230-H12 interval. The physical distance between the two markers was 29.7 kb and the genetic distance was about 0.3 cM. The results could lay the foundation for the further cloning of Ty-3a gene and molecular marker-assisted selection of new tomato varieties against Tomato yellow leaf curl.