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目的:研究Bcl-3蛋白在结直肠癌组织中的表达状况,以及其与结直肠癌临床特征和病理分期分级的关系。方法:采用免疫组织化学方法测定46例结直肠癌手术标本中癌组织、癌旁组织和正常肠组织的Bcl-3表达状况,分析其表达状况和患者年龄、性别、病理分期及病理分级的相关性。结果:免疫组织化学结果表明,肠癌组织、癌旁组织和正常肠组织中Bcl-3核表达的阳性率分别为76.1%(35/46)、58.7%(27/46)和28.3%(13/46),差异有统计学意义,χ2=21.730,P=0.000。Bcl-3核表达在低分化结直肠癌患者的阳性率为90.5%(19/21),在中高分化结直肠癌患者的阳性率为64.0%(16/25),差异有统计学意义,χ2=4.397,P=0.036。Bcl-3的核表达阳性率与结直肠癌患者的年龄、性别、病理分期无相关性,P>0.05。结论:与癌旁组织及正常肠组织相比,Bcl-3显著高表达于结直肠癌组织的细胞核内,而且核表达水平与细胞分化程度显著相关。
OBJECTIVE: To study the expression of Bcl-3 protein in colorectal cancer and its relationship with the clinical features and histological grade of colorectal cancer. Methods: Immunohistochemistry was used to determine the expression of Bcl-3 in 46 cases of colorectal cancer specimens, adjacent tissues and normal tissues. The expression of Bcl-3 was analyzed by age, gender, pathological stage and pathological grade Sex. Results: Immunohistochemistry results showed that the positive rates of Bcl-3 in colorectal cancer tissues, paracancerous tissues and normal intestinal tissues were 76.1% (35/46), 58.7% (27/46) and 28.3% (13.3%) respectively / 46), the difference was statistically significant, χ2 = 21.730, P = 0.000. The positive rate of Bcl-3 nuclear expression in poorly differentiated colorectal cancer was 90.5% (19/21), and the positive rate of Bcl-3 in patients with moderately well differentiated colorectal cancer was 64.0% (16/25), the difference was statistically significant (χ2 = 4.397, P = 0.036. There was no correlation between the positive rate of Bcl-3 expression and the age, sex and pathological stage of colorectal cancer (P> 0.05). CONCLUSION: Bcl-3 is significantly higher expressed in the nucleus of colorectal cancer than that in paracancerous tissues and normal intestinal tissues, and the expression of Bcl-3 is significantly correlated with the degree of cell differentiation.