论文部分内容阅读
目的观察单壁碳纳米管(single-walled carbon nanotube,SWCNT)对大鼠肝细胞株(BRL)的体外细胞毒性。方法选择正常大鼠肝细胞株BRL,分别以剂量5、30和60μg/ml染毒,应用MTT法检测染毒12、24、36、48、60和72 h后对细胞存活率的影响;利用倒置显微镜观察染毒48 h后细胞形态学改变;并检测染毒48 h后细胞中谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)、超氧化物歧化酶(Superoxide dismutase,SOD)活性和丙二醛(Malonaldehyde,MDA)含量;采用流式细胞术(Flow cytometer,FCM)检测SWCNT对细胞凋亡的影响。结果 SWCNT可引起BRL细胞结构异常;可影响BRL细胞增殖,且随着染毒剂量和染毒时间的增加而逐渐增加。染毒48 h后,随着SWCNT染毒剂量的增加,BRL细胞中SOD、GSH-Px活力逐渐降低,MDA含量逐渐增加,细胞凋亡率逐渐升高,且与对照组相比差异均有统计学意义。结论 SWCNT可对BRL细胞产生一定的损伤并能诱导细胞凋亡,氧化性损伤可能是其毒作用机制之一。
Objective To observe the in vitro cytotoxicity of single-walled carbon nanotube (SWCNT) on rat hepatocyte cell line (BRL). Methods Normal rat hepatocyte cell line BRL was selected and exposed to doses of 5, 30 and 60 μg / ml respectively. The cell viability was measured by MTT assay at 12, 24, 36, 48, 60 and 72 h. The morphological changes of cells were observed by inverted microscope 48 hours after exposure. The activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) And malonaldehyde (MDA). The effect of SWCNT on apoptosis was detected by flow cytometer (FCM). Results SWCNT caused structural abnormalities of BRL cells; it could affect the proliferation of BRL cells, and gradually increased with the increase of exposure dose and exposure time. At 48 h after exposure, the activities of SOD and GSH-Px in BRL cells gradually decreased, the content of MDA gradually increased, and the apoptosis rate increased gradually with the increase of the dose of SWCNT, and the difference was statistically significant compared with the control group Significance of learning. Conclusion SWCNT can induce BRL cell injury and induce apoptosis. Oxidative damage may be one of the mechanisms of its toxicity.