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目的 观察甘草提取液对糖尿病大鼠肾脏的保护作用及其机制。方法 Wistar大鼠48只随机分为对照组、模型组和治疗组,每组16只。于第8周及第12周测定各组大鼠的肾质量指数(KI)、葡萄糖氧化酶法测定空腹血糖(FBG)、微柱法测定糖化血红蛋白(GHbA1c)、考马斯亮兰法测定24h尿白蛋白量、速率法测定血和尿肌酐、硫代巴比妥酸法测定血液及肾组织匀浆中丙二醛(MDA)含量、黄嘌呤氧化酶法测定血液及肾组织匀浆中超氧化物歧化酶(SOD)活性、比色法测定血液及肾组织匀浆中硒谷胱甘肽过氧化物酶(SeGSHPx)和醛糖还原酶(AR)活性、计算内生肌酐清除率(Ccr)。结果 与对照组相比,模型组及治疗组所有指标的差异均有统计学意义(P均<0.05)。与模型组相比,治疗组KI、FBG、GHbA1c、24h尿白蛋白量、Ccr、血液及肾组织匀浆中MDA含量、AR活性均显著降低(P均<0.05),SOD、SeGSHPx活性均显著升高(P均<0.05)。结论 甘草提取液对糖尿病大鼠具有肾脏保护作用,其机制与调节血糖、降低肾脏AR活性、提高肾脏抗氧化能力有关。
Objective To observe the protective effect of licorice extract on the kidney of diabetic rats and its mechanism. Methods 48 Wistar rats were randomly divided into control group, model group and treatment group, 16 in each group. At the 8th week and the 12th week, the rats’ kidney mass index (KI), the fasting blood glucose (FBG) by the glucose oxidase method, the glycated hemoglobin (GHbA1c) by microcolumn method and the urinary white urine by Coomassie brilliant blue method The contents of malondialdehyde (MDA) in blood and kidney homogenates were determined by thiobarbituric acid method. The levels of malondialdehyde (MDA) in blood and renal homogenates were measured by xanthine oxidase method. The levels of superoxide dismutase The activities of SOD and Se activity and the activity of selenium glutathione peroxidase (SeGSHPx) and aldose reductase (AR) in blood and kidney homogenates were measured by colorimetric method. The creatinine clearance rate (Ccr) was calculated. Results Compared with the control group, the differences between the model group and the treatment group were statistically significant (all P <0.05). Compared with model group, the contents of KI, FBG, GHbA1c, urinary albumin, Ccr, MDA content and AR activity in blood and kidney homogenate were significantly decreased (all P <0.05), while the activities of SOD and SeGSHPx were significantly Increased (P <0.05). Conclusion Glycyrrhiza uralensis has protective effect on diabetic rats, and its mechanism is related to the regulation of blood glucose, reducing the renal AR activity and increasing the renal antioxidant capacity.