Involvement of Ca~(2+) activated K~+channels in receptor regulated sperm motility in rats

来源 :Asian Journal of Andrology | 被引量 : 0次 | 上传用户：wcp

【摘要】

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Objective: To investigate the involvement of KCa channels in receptor-regulated sperm motility of the rat. Methods: Sperm motility was studied using a computer-

【出处】

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Asian Journal of Andrology

【发表日期】

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2003年01期

【关键词】

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sperm activated K motility

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Objective: To investigate the involvement of KCa channels in receptor-regulated sperm motility of the rat. Methods: Sperm motility was studied using a computer-aided sperm analysis system, HTM-IVOS, in conjunction with Ca2+-mobilizing agents, receptor agonists/antagonists and KCa channels blockers. Results: The percentage of motile sperm was increased by ionomycin (0.5μmol/L), which could be inhibited by K+ channel blockers, tetraethylammonium (TEA 1μmol/L ) or charybdotoxin (ChTX, 300 nmol/L) indicating the presence of KCa channels. Ang Ⅱ, at low concentration, 10 nmol/L, was found to increase motility, however, at higher concentration, 1 μmol/L, percentage of motility was found to be suppressed. Both stimulatory and inhibitory effects of Ang Ⅱ could be reversed by losartan, a specific antagonist of AT1 receptors, but not AT2 antagonist PD123177, indicating the involvement of AT1 but not AT2 receptor in mediating both effects. ChTX also abolished both the stimulatory and inhibitory effects of Ang Ⅱ, suggesting the involvement of KCa channels. The percentage of motility was also enhanced by extracellular ATP, a factor known to be involved in sperm activation. The ATP-enhanced sperm motility was mimicked by UTP and inhibited by ChTX and reactive blue, an antagonist of P2 receptor, indicating the involvement of both P2U and KCa channels. RT-PCR study was also conducted to confirm the expression of KCa channels, AT1 receptors and P2U receptor, but not AT2 receptor, in rat caudal epididymal sperm. Conclusion: KCa channels may play an important role in the regulation of sperm motility by AT1 and P2U receptors. Objective: To investigate the involvement of KCa channels in receptor-regulated sperm motility of the rat. Methods: Sperm motility was studied using a computer-aided sperm analysis system, HTM-IVOS, in conjunction with Ca2 + -mobilizing agents, receptor agonists / antagonists and KCa channels blockers. Results: The percentage of motile sperm was increased by ionomycin (0.5 μmol / L), which could be inhibited by K + channel blockers, tetraethylammonium (TEA 1 μmol / L) or charybdotoxin (ChTX, 300 nmol / L) indicating the presence of KCa channels. Ang Ⅱ, at low concentration, 10 nmol / L, was found to increase motility, however, at higher concentration, 1 μmol / L, percentage of motility was found to be suppressed. inhibitory effects of Ang II could be reversed by losartan, a specific antagonist of AT1 receptors, but not AT2 antagonist PD123177, indicating the involvement of AT1 but not AT2 receptor in mediating both effects. ChTX also abolished both the stimulatory and inhibitory effects of Ang Ⅱ, suggesting the involvement of KCa channels. The percentage of motility was also enhanced by extracellular ATP, a factor known to be involved in sperm activation. The ATP-enhanced sperm motility was mimicked by UTP and inhibited by ChTX and reactive blue, an antagonist of P2 receptor, indicating the involvement of both P2U and KCa channels. RT-PCR study was also conducted to confirm the expression of KCa channels, AT1 receptors and P2U receptor, but not AT2 receptor, in rat caudal epididymal sperm Conclusion: KCa channels may play an important role in the regulation of sperm motility by AT1 and P2U receptors.

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Involvement of Ca~(2+) activated K~+channels in receptor regulated sperm motility in rats

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