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目的探讨子宫颈病变中HPVL1蛋白的阳性表达与HPVDNA感染亚型的关系。方法选取已知HPVDNA感染阳性患者的宫颈细胞学标本145例,采用以免疫组织/细胞化学和非同位素标记核酸分子杂交技术为基础,在免疫水平和核酸水平上对其进行HPVL1蛋白检测。结果 HPVL1蛋白总阳性率为44.14%(64/145),其中HPV混合感染型的HPVL1蛋白的阳性率57.63%(34/59),高于HPV单一感染型34.88%(30/86),两者比较差异有统计学意义(χ2=7.34,P<0.05)。HPVL1蛋白的阳性表达在不同年龄段之间的无显著差异,在40岁以下的阳性率为33.85%(22/65),41~50岁的阳性率为50.94%(27/53),50岁以上的患者阳性率为55.56%(15/27)两者比较差异无统计学意义(χ2=5.22,P>0.05)。HPV感染亚型的HPVL1蛋白的阳性率分别为:HPV56型68.75%(11/16),HPV35型66.67%(2/3),HPV33型66.67%(14/21),HPV11型60.00%(3/5),HPV53型52.38%(11/21),HPV43型50.00%(2/4),HPV59型50.00%(1/2),HPV58型44.00%(11/25),HPV6型40.00%(2/5),HPV52型37.14%(13/35),HPV45型33.33%(1/3),HPV16型29.03%(9/31),HPV68型25.00%(2/8),HPV18型20.00%(3/15),HPV31型14.29%(1/7),HPV66型12.50%(1/8),HPV55型0.00%(0/2),HPV44型0.00%(0/1)。在HPV感染亚型中HPV56型HPVL1蛋白阳性率明显高于HPV44型,两者之间差异有统计学意义(χ2=6.75,P<0.05)。结论 HPVL1蛋白的阳性表达与患者的年龄无关,与HPV感染亚型密切相关,HPVL1蛋白联合HPVDNA亚型的检测对宫颈病变的治疗具有重要指导意义,这二者可以作为宫颈病变患者的常规检测指标,HPVL1蛋白的检测对感染HPVDNA的患者的治疗及预后的评估提供了可靠的依据,具有重要的指导意义及临床应用价值。
Objective To investigate the relationship between the positive expression of HPV L1 protein and the subtype of HPVDNA in cervical lesions. Methods A total of 145 cervical cytology specimens from patients with known HPVDNA infection were selected for detection of HPVL1 protein at the immunological level and nucleic acid level based on the hybridization of immunohistochemistry / cytochemistry and non-isotope labeled nucleic acid. Results The positive rate of HPV L1 protein was 44.14% (64/145). The positive rate of HPV L1 protein was 57.63% (34/59), higher than that of HPV single infection 34.88% (30/86) The difference was statistically significant (χ2 = 7.34, P <0.05). The positive expression rate of HPV L1 protein in different age groups had no significant difference. The positive rate of HPV L1 protein was 33.85% (22/65) under 40 years old, the positive rate was 50.94% (27/53) between 41-50 years old and 50 years old The positive rate of these patients was 55.56% (15/27). There was no significant difference between them (χ2 = 5.22, P> 0.05). The positive rates of HPV L1 protein in HPV subtypes were 68.75% (11/16) for HPV56, 66.67% (2/3) for HPV35, 66.67% (14/21) for HPV33 and 60.00% for HPV11 5 of HPV type 5, 50.00% (2/4) of HPV43 type, 50.00% (1/2) of HPV59 type, 44.00% (11/25) of HPV58 type and 40.00% (2 / 5 HPV26 type HPV infection and HPV type 18 virus infection rate of HPV18 type HPV infection, 15), HPV29 (14.29% (1/7)), HPV66 12.50% (1/8), HPV55 0.00% (0/2) and HPV44 0.00% (0/1). The positive rate of HPV56 HPV L1 protein in HPV subtype was significantly higher than HPV44 type (χ2 = 6.75, P <0.05). Conclusion The positive expression of HPV L1 protein has nothing to do with the patient’s age and is closely related to the subtype of HPV infection. The detection of HPV L1 protein combined with HPVDNA subtypes has important guiding significance for the treatment of cervical lesions, both of which can be used as routine detection indicators of cervical lesions The detection of HPV L1 protein provides a reliable basis for the evaluation of the treatment and prognosis of patients with HPVDNA infection, which has important guiding significance and clinical value.