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目的监测糖尿病患者活动性人巨细胞病毒(HCMV)感染,并探讨其临床意义。方法在HCMV pp65基因内自行设计一对引物,建立RT-PCR检测727例糖尿病患者及230名健康献血者外周血白细胞HCMVpp65 mRNA转录水平,同时应用抗体捕获酶联免疫法检测HCMV pp65-IgM。结果糖尿病患者HCMV-IgM及mRNA阳性率分别为11.14%和16.64%,与对照组(0.87%、2.17%)相比均差异有统计学意义(P<0.01);比较不同年龄段HCMV感染率,结果发现发病年龄<30岁的患者中HCMV-mRNA阳性率明显高于其他年龄组,从病程来看,病程<1年的糖尿病患者HCMV-mRNA阳性检出率最高。结论糖尿病患者易发生活动性的HCMV感染,RT-PCR检测HCMV pp65和抗体捕获HCMV pp65特异性IgM可快速有效地确诊HCMV活动性感染的发生。
Objective To monitor active human cytomegalovirus (HCMV) infection in patients with diabetes mellitus and to explore its clinical significance. Methods A pair of primers was designed in HCMV pp65 gene. RT-PCR was used to detect the mRNA level of HCMVpp65 mRNA in peripheral blood leukocytes of 727 diabetic patients and 230 healthy blood donors. HCMV pp65-IgM was detected by enzyme-linked immunosorbent assay (ELISA). Results The positive rates of HCMV-IgM and mRNA in diabetic patients were 11.14% and 16.64%, respectively, which were significantly different from those in the control group (0.87%, 2.17%, P <0.01). The HCMV infection rates, The results showed that the positive rate of HCMV-mRNA in patients with onset age <30 years was significantly higher than that in other age groups. From the course of disease, HCMV-mRNA positive rate was the highest in patients with diabetes <1 year. Conclusions HCMV infection is more likely to occur in diabetic patients. The detection of HCMV pp65 by RT-PCR and pp65-specific IgM by antibody capture can be used to confirm the occurrence of active HCMV infection quickly and effectively.