论文部分内容阅读
【目的】了解各个发育时期控制水稻分蘖数QTL的“静态”和“动态”信息。【方法】利用单片段代换系对不同时期的水稻分蘖数QTL同时进行非条件和条件定位分析。【结果】(1)水稻分蘖数至少受14个QTL影响,它们分布在第1、2、3、4、6、7和8号共7条染色体的相应代换片段上;(2)各个时期影响水稻分蘖数的QTL数量(变动在6～9之间)和效应(变动在1.49～3.49之间)均不相同;(3)水稻分蘖数QTL的表达具有很强的时序性,主要集中在移栽后0～7d(有6个正表达),14～21d(有9个随机表达)和35～42d(有6个负表达)3个时间段内,正、负表达分别决定了最高分蘖和有效分蘖的数量;(4)每个QTL在整个生育期至少表达1次,有些可多次表达;(5)某个时期的分蘖数量取决于所有QTL的累积效应,而某个时间段内分蘖数的增减量则取决于所有QTL的净效应。【结论】用单片段代换系和条件QTL定位方法对发育性状进行QTL定位分析非常有效和准确。
【Objective】 To understand the “static” and “dynamic” information of QTL controlling tillering number in different developmental stages. 【Method】 The QTLs for tillering number of rice at different stages were simultaneously analyzed under both non-conditional and conditional loci using single-fragment substitution lines. 【Result】 (1) The number of tillers in rice was affected by at least 14 QTLs, which were distributed on the corresponding substitutions of 7 chromosomes on chromosomes 1, 2, 3, 4, 6, 7 and 8. (2) The number of QTL affecting tillering number of rice varied from 6 to 9 and the effect varied from 1.49 to 3.49. (3) The expression of tillering number QTL in rice was highly time-dependent Within transplanting 0 ~ 7d (6 positive expression), 14 ~ 21d (9 random expression) and 35 ~ 42d (6 negative expression) three time periods, positive and negative expression of the highest tiller And the number of effective tillers. (4) Each QTL was expressed at least once during the whole growth period, some of which could be expressed more than once. (5) The number of tillers at a certain period was dependent on the cumulative effect of all QTLs. The increase or decrease in tiller number depends on the net effect of all QTLs. 【Conclusion】 It is very effective and accurate to conduct QTL mapping of developmental traits by single-locus substitution lines and conditional QTL mapping.