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为进一步提高骨修复材料的骨诱导性能,引入了BaTiO_3压电涂层。首先,通过等离子喷涂法在Ti基体上制备羟基磷灰石(HA)、Ti-HA和Ti-HA-BaTiO_3这3种生物涂层;然后,采用正交试验设计优化了喷涂工艺参数,用SEM/EDS对涂层的形貌和成分进行了表征,并通过划痕试验测试了涂层与基体的结合强度;最后,进行了细胞培养试验,用CCK-8法对细胞毒性进行了评价,并在SEM下观察了细胞的粘附形态。结果显示:细胞带有伪足,呈梭形和不规则多边形,且粘附在Ti-HA-BaTiO_3复合涂层表面;细胞伸展形态良好,涂层的细胞毒性等级不高于1级。可见,Ti-HA-BaTiO_3复合材料可以作为临床植入材料应用。
To further improve the osteoinductive properties of bone repair materials, BaTiO_3 piezoelectric coatings were introduced. Firstly, hydroxyapatite (HA), Ti-HA and Ti-HA-BaTiO_3 were prepared on Ti substrate by plasma spraying. Then, the coating parameters were optimized by orthogonal design. / EDS were used to characterize the morphology and composition of the coating. The adhesion of the coating to the matrix was tested by scratch test. Finally, cell culture experiments were performed to evaluate the cytotoxicity by CCK-8 method. Cell adhesion morphology was observed under SEM. The results showed that the cells were pseudopodia, spindle-shaped and irregular polygons, which adhered to the surface of Ti-HA-BaTiO_3 composite coating. The cell morphology was good and the cytotoxicity of the coating was not higher than 1 grade. Can be seen, Ti-HA-BaTiO_3 composite materials can be used as clinical implants.