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成年大鼠,雌,背部用沸水烫伤,轻烫组的烫伤面积为5.3cm直径的圆面积。烫10秒。重烫组为6.5cm直径,15秒。于烫伤后1、3、6和9小时断头处死,测定血浆皮质酮(B)、肝胞浆糖皮质激素受体(GCR)和酪氨酸转氨酶活性(TAT)。结果烫后3和6小时,尽管重烫组血浆B很高,但TAT却明显低于轻烫组(P<0.001),反映了严重烫伤组靶细胞对内源性糖皮质激素(GC)的反应性降低。烫后1、3小时重烫组GCR的结合容量(R_0)也明显低于轻烫组,提示重烫组靶细胞对GC的反应性降低至少部分地是由于GCR减少所致。对严重烫伤时靶细胞对GC的反应性降低的可能的病理和临床意义作了讨论。
Adult rats, female and back scalded with boiling water, scald group scald area of 5.3cm diameter circular area. Hot 10 seconds. Heavy-hot group 6.5cm diameter, 15 seconds. At 1, 3, 6, and 9 hours after scald decapitation, plasma corticosterone (B), hepatic cytoplasmic glucocorticoid receptor (GCR) and tyrosine aminotransferase activity (TAT) were measured. Results TAT was significantly lower at 3 and 6 h postburn than in the scalded group (P <0.001), although the plasma B was higher in the hyperbollic group, reflecting the effect of target cells on endogenous glucocorticoid (GC) Reactivity decreased. The binding capacity (R_0) of GCR in the scalding group was also significantly lower than that of the scalding group at 1 and 3 h after scalding, suggesting that the reactivity of target cells in scalding group to GC was at least partially attributed to the decrease of GCR. The possible pathological and clinical implications of the target cell response to GC at severe scald are discussed.