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目的探讨出生前邻苯二甲酸二(2-乙基己基)酯(DEHP)暴露对成年后SD大鼠生殖腺促性腺激素释放激素(GnRH)、促性腺激素释放激素受体(GnRHR)mRNA表达水平的影响。方法将32只健康SD孕鼠随机分为0(溶剂对照组)和2、10、50 mg/kg DEHP染毒组,每组8只。于妊娠第14~19天,采用灌胃方式进行染毒,染毒容量为10 ml/kg,每天1次。仔鼠出生后正常饲养10周,采用实时荧光定量PCR检测卵巢和睾丸GnRH及GnRHR mRNA的表达水平。结果与对照组相比,仅2 mg/kg DEHP染毒组成年雌性仔鼠卵巢组织内的GnRH mRNA表达水平升高,差异有统计学意义(P<0.05);而各剂量DEHP染毒组成年雌性仔鼠卵巢组织内GnRHR mRNA的表达水平均无明显改变。各剂量DEHP染毒组成年雄性仔鼠睾丸组织GnRH及GnRHR mRNA的表达水平与对照组相比,差异均无统计学意义(P>0.05)。结论出生前低剂量DEHP暴露可能通过上调成年后子代雌鼠卵巢GnRH基因表达水平,干扰雌性生殖内分泌调控功能,且可能存在低剂量效应。
Objective To investigate the effect of prenatal exposure to DEHP on the expression of gonadotropin-releasing hormone (GnRH), gonadotropin-releasing hormone receptor (GnRHR) mRNA in adult gonads of SD rats Impact. Methods Thirty-two healthy SD pregnant rats were randomly divided into 0 (solvent control group) and 2, 10 and 50 mg / kg DEHP exposure groups, 8 in each. On the 14th to 19th day of gestation, the rats were drenched by gavage with the capacity of 10 ml / kg once a day. After birth, the offspring were normally fed for 10 weeks. The mRNA expression of GnRH and GnRHR in ovary and testis were detected by real-time fluorescence quantitative PCR. Results Compared with the control group, the expression level of GnRH mRNA in the ovaries of female offspring of only 2 mg / kg DEHP exposed group was significantly higher than that of the control group (P <0.05) There was no significant difference in the expression of GnRHR mRNA in ovary of female offspring. Compared with the control group, the expression levels of GnRH and GnRHR mRNA in testis tissue of male offspring rats in each dose of DEHP exposure group showed no significant difference (P> 0.05). Conclusions Low-dose DEHP exposure may interfere with female reproductive and endocrine regulation by up-regulating the expression of GnRH gene in adult ovary female offspring, and there may be a low dose effect.