目的:采用HPLC法同时测定复方丹参输卵管灌注液中丹参素和原儿茶醛的含量。方法:采用Inertsil C_(18)(250 mm×4.6 mm,5μm)色谱柱;以甲醇-1%冰醋酸为流动相梯度洗脱;流速为1.0 ml·min~(-1);检测波长280 nm。结果:丹参素和原儿茶醛分别在9.8～78.2μg·ml~(-1)(r=0.999 9)、2.0～19.9μg·ml~(-1)范围内线性关系良好(r=0.999 6);加样回收率分别为98.59%和100.20%,RSD分别为0.25%和0.78%。结论:该方法简单可行、灵敏准确,可用于复方丹参输卵管灌注液的质量控制。 Objective: To determine the contents of Danshensu and protocatechuic aldehyde in Compound Salviae miltiorrhizae by HPLC method. METHODS: Inertsil C 18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was eluted with methanol-1% glacial acetic acid. The flow rate was 1.0 ml · min -1. The detection wavelength was 280 nm . Results: There was a good linear relationship between Danshensu and protocatechuic aldehyde in the range of 9.8 ~ 78.2μg · ml ~ (-1) (r = 0.999 9) and 2.0 ~ 19.9μg · ml ~ (-1) (r = 0.999 6 ). The average recoveries were 98.59% and 100.20%, respectively, with RSDs of 0.25% and 0.78%, respectively. Conclusion: The method is simple and feasible, sensitive and accurate, and can be used for the quality control of Salvia miltiorrhiza tubal perfusate.