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目的:研究细胞周期蛋白D1(CCND1)基因第4外显子870位点单核苷酸多态性与卵巢癌遗传易感性的关系。方法:采用分子流行病学病例-对照研究,病例组为107例经病理组织学确诊的新发卵巢癌患者,同时按年龄相匹配选取114例正常对照者,使用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测病例与对照的基因型,比较不同基因型与卵巢癌发病危险性之间的关系,分层分析年龄、初潮年龄、初次生育年龄、产次、流产数及是否绝经对罹患卵巢癌风险的影响。结果:病例与对照之间的等位基因频率无统计学差异,本研究未发现CCND1G870A多态性与卵巢癌之间存在显著性相关。结论:CCND1G870A多态性与中国人群卵巢癌的发生未见明显相关。
Objective: To investigate the relationship between single nucleotide polymorphism (SNP) of exon 4 of CCND1 gene and genetic predisposition of ovarian cancer. Methods: A case-control study of molecular epidemiology was performed in 107 cases of newly diagnosed ovarian cancer patients by histopathology. At the same time, 114 normal controls were selected by age and matched with polymerase chain reaction-restriction fragment Length polymorphism (PCR-RFLP) method was used to detect genotypes of cases and controls. The relationship between different genotypes and the risk of ovarian cancer was compared. The results of stratified analysis of age, initial menarche, first childbearing age, parity and miscarriage Number and whether menopause affect ovarian cancer risk. Results: There was no significant difference in allele frequency between cases and controls. No significant association was found between CCND1G870A polymorphism and ovarian cancer. Conclusion: There is no significant correlation between CCND1G870A polymorphism and ovarian cancer in Chinese population.