青霉素皮试注射液是各医院必备而又常用的自制制剂。由于它含量低,期限较短,目前对青霉素测定的化学方法及微生物法都不能达到微量及快速的要求。笔者利用青霉素在咪唑的催化下,在氯化汞溶液中能定量地形成青霉素酸硫醇汞盐,并有稳定的紫外吸收的原理,对青霉素皮试注射液进行含量测定。作如下实验。 Penicillin skin test injection is essential and commonly used in various hospitals homemade preparations. Because of its low content, short duration, the current method of determination of penicillin and microbiological methods can not achieve trace and rapid requirements. I use penicillin in the imidazole catalysis, mercury chloride solution can quantitatively form penicillin acid mercaptide mercuric salt, and a stable UV absorption principle, penicillin skin test injection content determination. Make the following experiment