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目的:研究草豆蔻Alpinia katsumadai乙酸乙酯部位化学成分及其体外抗肿瘤作用。方法:采用多种柱层析色谱技术进行分离纯化,通过光谱和波谱分析鉴定化合物结构;采用MTT法测定化合物对Bel7402和L0-2细胞增殖的抑制作用。结果:分离鉴定了10个化合物,分别为:桤木酮(alnustone)、豆蔻明(cardamonin)、乔松素(pinocembrin)、蜡菊亭(helichrysetin)、山姜素(alpinetin)、反式桂皮酸(trans-cinnamicacid)、原儿茶酸(protocatechuic acid)、对羟基苯甲酸(p-hydroxybenzoic acid)、香草酸(vanillic acid)、β-谷甾醇(β-sitoserol)。体外抗肿瘤活性筛选结果显示,在浓度为25μmol/L时,化合物1对Bel 7402和L0-2细胞增殖的抑制率分别为62.61%和68.86%。结论:化合物反式桂皮酸、原儿茶酸、对羟基苯甲酸、香草酸为首次从该植物中分离得到;化合物桤木酮具有显著的抑制Bel 7402和L0-2细胞增殖作用。
Objective: To study the chemical constituents of Alpinia katsumadai ethyl acetate and the antitumor effects in vitro. Methods: The compounds were isolated and purified by column chromatography. The structures of the compounds were identified by spectral and spectral analysis. The inhibitory effects of the compounds on the proliferation of Bel7402 and L0-2 cells were determined by MTT assay. Results: Ten compounds were isolated and identified as alnustone, cardamonin, pinocembrin, helichrysetin, alpinetin, trans- cinnamic acid, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, and β-sitoserol. The in vitro antitumor activity screening results showed that the inhibitory rates of compound 1 on the proliferation of Bel 7402 and L0-2 cells were 62.61% and 68.86% at a concentration of 25 μmol / L, respectively. CONCLUSION: The compounds trans-cinnamic acid, protocatechuic acid, p-hydroxybenzoic acid and vanillic acid were isolated from this plant for the first time. Compound almond ketone has a significant inhibitory effect on the proliferation of Bel 7402 and L0-2 cells.