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目的比较酶消化法与组织块法培养大鼠视网膜Müller细胞的效果。方法将新生3d的SD大鼠视网膜分离为小组织块,分别采用酶消化法与组织块法进行体外培养,采用换液时机械震荡等方法获得纯化的Müller细胞。从细胞形态学、细胞增殖及免疫细胞化学染色阳性率方面比较两种细胞培养方法的效果。结果酶消化法获得的Müller细胞数量多于组织块法(t=2.264,P<0.05),且用时短。两种方法培养的纯化Müller细胞增殖速度及免疫细胞化学染色阳性率比较差异无显著意义(P>0.05)。结论酶消化法是一种高效的大鼠视网膜Müller细胞培养方法。
Objective To compare the effects of enzymatic digestion and tissue culture on rat retinal Müller cells. Methods Retina of neonatal SD rats were separated into small pieces of tissue, and cultured in vitro by enzymatic digestion and tissue block method respectively. The purified Müller cells were obtained by mechanical shaking during liquid exchange. The effects of two cell culture methods were compared in terms of cell morphology, cell proliferation, and immunocytochemical staining positive rates. Results The number of Müller cells obtained by enzymatic digestion was more than that of tissue block method (t = 2.264, P <0.05), and the time was short. The proliferation rate of purified Müller cells cultured by the two methods and the positive rate of immunocytochemical staining showed no significant difference (P> 0.05). Conclusion Enzymatic digestion is an efficient method of retinal Müller cell culture in rats.