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为了解萍乡地区肝炎病毒在人群中感染分布状况，分析肝病病毒株基因特点，采用 Ｐ Ｃ Ｒ 方法检测血清标本中 Ｔ Ｔ Ｖ－ Ｄ Ｎ Ａ，对 Ｔ Ｔ Ｖ－ Ｄ Ｎ Ａ 阳性标本进行了序列测定，分析其基因变化情况。结果显示在９０ 例就诊患者血清标本中， Ｔ Ｔ Ｖ－ Ｄ Ｎ Ａ 阳性率为３３３ ％ ， Ａ Ｌ Ｔ 增高３９ 例病人中， Ｔ Ｔ Ｖ－ Ｄ Ｎ Ａ 阳性率为４３６ ％ ， Ａ Ｌ Ｔ 正常４６ 例中， Ｔ Ｔ Ｖ－ Ｄ Ｎ Ａ 的阳性率为１９６ ％ ，２８ 例献血员中， Ｔ Ｔ Ｖ－ Ｄ Ｎ Ａ 阳性率为２５ ％ 。对其中两株病毒 Ｄ Ｎ Ａ 片段进行序列测定。该序列与日本株（ Ａ Ｂ００８３９４） 同源性为７５４ ％ 和７６８ ％ ，与日本株（ Ｎ Ａ００４） 同源性为８８６ ％ 和８３３ ％ ，与 Ｔ Ｔ Ｖ Ｓ Ｚ１ 和 Ｔ Ｔ Ｖ Ｓ Ｚ２ 同源性分别是７３９ ％ 、７３２ ％ 和７５７ ％ 、７４３ ％ ， Ｊ Ｘ１ 和 Ｊ Ｘ２ 同源性为８１７４ ％ 。说明萍乡地区存在 Ｔ Ｔ Ｖ 感染，本地流行毒株可能是 Ｇ２ 亚型。
In order to understand the distribution of hepatitis B virus infection in the population in Pingxiang and to analyze the gene characteristics of the liver disease virus strains, T T V-D N A in serum samples were detected by P C R method, and T V-D N A positive samples were sequenced Determination, analysis of its genetic changes. The results showed that the positive rate of T V-D N A was 333% in the serum samples from 90 patients, and the positive rate of T V-D N A was 436% in 39 patients with elevated A L T, The positive rate of T T V-D N A was 196% in 46 normal cases of ALT. The positive rate of T V-D N A was 25% in 28 cases of blood donors. Two of the virus DNA fragments were sequenced. The sequence was 754% and 768% homologous with Japanese strain (A B008394), 886% and 833% homologous with Japanese strain (N A004) The homologies with T T V S Z2 were 739%, 732% and 757%, 743%, respectively. The homologies of J X1 and J X2 were 8174%. TTV infection in Pingxiang shows that the endemic strain may be G2 subtype.