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为了确定通过丙型肝炎病毒(HCV)抗体(抗-HCV)筛选法排除HCV阳性供血员的有效率,作者采用聚合酶链反应(PCR)对来自6个制造厂的抗-HCV阴性的混合血浆和血液制品进行HCV RNA检测.RNA从标本中抽提,用HCV基因组5’非编码区的一对引物进行扩增.
To determine the efficiency of excluding HCV-positive blood donors by the Hepatitis C virus (HCV) antibody (anti-HCV) screening method, the authors used polymerase chain reaction (PCR) to measure anti-HCV negative mixed plasma And blood products for HCV RNA detection.RNA was extracted from the specimens and amplified with a pair of primers in the 5 ’non-coding region of the HCV genome.