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目的:研究地黄多糖(RPS)对K562细胞bcr/abl融合基因表达和细胞增殖的影响。方法:不同浓度RPS作用于K562细胞,MTS法检测其对细胞增殖的影响,Real-time PCR检测bcr/abl融合基因mRNA表达变化,Western blotting检测bcr/abl融合基因表达的P210蛋白变化。结果:RPS能够抑制K562细胞增殖,用400μg·mL-1 RPS作用12 h,细胞增殖抑制率为35.16%,随着RPS浓度增加抑制作用明显增强(P<0.05);用200μg·mL-1 RPS作用24 h,K562细胞内bcr/abl融合基因mRNA相对表达量为0.26,P210蛋白相对含量为0.35。RPS使K562细胞内P210蛋白及bcr/abl融合基因mRNA表达量呈时间和剂量依赖性下降。结论:RPS可能通过下调bcr/abl融合基因mRNA及P210蛋白表达对K562细胞增殖产生抑制作用。
Objective: To study the effect of RPS on bcr / abl fusion gene expression and cell proliferation in K562 cells. Methods: K562 cells were treated with different concentrations of RPS. The cell proliferation was measured by MTS method. The mRNA expression of bcr / abl fusion gene was detected by Real-time PCR. The expression of b10 protein was detected by Western blotting. Results: RPS could inhibit the proliferation of K562 cells. The inhibitory rate of RPS was 35.16% after treated with 400μg · mL-1 RPS for 12h, and the inhibitory effect was enhanced with the increase of RPS concentration (P <0.05) After 24 h, the relative expression of bcr / abl fusion gene mRNA in K562 cells was 0.26, and the relative content of P210 protein was 0.35. RPS decreased the expression of P210 protein and bcr / abl fusion gene mRNA in K562 cells in a dose- and time-dependent manner. Conclusion: RPS may inhibit the proliferation of K562 cells by down-regulating bcr / abl fusion gene mRNA and P210 protein expression.