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[目的]观察亚致死性浓度的二噁英(TCDD)对斑马鱼胚胎发育的毒性作用。[方法]将72hpf(受精后小时)斑马鱼胚胎暴露于渐升的TCDD亚致死性浓度下,观察累积死亡率、心功能及颌软骨发育的变化,与对照组比较。[结果]10pg/ml及25pg/ml的TCDD浓度所导致的两周内的累积死亡率与对照组类似。然而在胚胎内这两种浓度的TCDD均能导致急性及亚急性心脏毒性。暴露在这两种浓度TCDD下与对照组相比,在毒物处理后的12h、1d、2d及1周后胚胎均表现出心每搏输出量及心输出量的显著降低。最显著的发现是72hpf胚胎在10pg/ml的TCDD处理后1周表现出显著的每搏输出量及心输出量降低,伴轻微的心包积液。[结果]该研究表明较低浓度的二噁英污染对野生鱼类能造成威胁,而亚致死性浓度的TCDD对胚胎的颌骨发育无影响。
[Objective] To observe the toxic effect of sub-lethal concentration of dioxin (TCDD) on zebrafish embryo development. [Method] The zebrafish embryos exposed to 72 hpf (hour after fertilization) were exposed to the elevated TCDD sublethal concentration. The cumulative mortality, cardiac function and the development of jaw cartilage were observed, compared with the control group. [Results] The cumulative death rates within two weeks caused by TCDD concentrations of 10 pg / ml and 25 pg / ml were similar to those of the control group. However, both concentrations of TCDD in embryos can cause both acute and subacute cardiotoxicity. Exposed to these two concentrations of TCDD compared with the control group, 12h, 1d, 2d and 1 week after poisoning embryos showed a significant decrease in cardiac output and cardiac output. The most striking finding was that 72 hpf embryos showed significant stroke volume and cardiac output one week after 10 pg / ml TCDD treatment, with mild pericardial effusion. [Results] The study showed that dioxin contamination at lower concentrations could pose a threat to wild fish, whereas sub-lethal concentrations of TCDD had no effect on embryonic jaw development.