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目的:探讨CD24,ALCAM在大肠癌中的表达及其与细胞增殖、血管生成的关系。方法:运用免疫组织化学检测66例大肠癌肿瘤组织CD24,ALCAM,CD34,PCNA的表达情况,根据CD34的染色情况计算出大肠癌组织的微血管密度(microvessel density,MVD),根据PCNA染色情况计算出肿瘤细胞PCNA标记指数。结果:免疫组织化学染色显示正常大肠黏膜CD24,ALCAM绝大部分呈阴性染色,超过75%的大肠癌细胞有不同程度的CD24染色。44%的肿瘤CD24呈中等强度表达,26%的肿瘤呈强阳性表达。高Dukes分期、高pTNM分期及有淋巴结转移、浆膜外浸润的癌组织中CD24蛋白染色强度显著高于对应组(P<0.01)。68%的大肠癌细胞有不同程度的AL-CAM染色。ALCAM表达与浸润深度、pTNM分期,淋巴结转移明显正相关。在CD24,ALCAM阳性表达病例中,PCNA的表达随着CD24表达强度的升高而升高(P<0.05)。大肠癌CD24蛋白表达强度与大肠癌微血管密度轻度相关(r=0.228,P=0.019)。大肠癌ALCAM蛋白质表达与MVD无关(P=0.17)。结论:CD24,ALCAM可能在大肠癌发生发展中起重要作用。
Objective: To investigate the expression of CD24 and ALCAM in colorectal carcinoma and its relationship with cell proliferation and angiogenesis. Methods: Immunohistochemistry was used to detect the expression of CD24, ALCAM, CD34 and PCNA in 66 cases of colorectal cancer. The microvessel density (MVD) of colorectal cancer was calculated according to the staining of CD34, Tumor cell PCNA labeling index. Results: Immunohistochemical staining showed that most of the normal colorectal mucosa CD24 and ALCAM negative staining, more than 75% of colorectal cancer cells have different degrees of CD24 staining. Forty-four percent of tumors showed moderate-intensity CD24 expression and 26% of tumors showed strong positive expression. The staining intensity of CD24 protein in high Dukes stage, high pTNM stage, lymph node metastasis and extra-serous infiltration was significantly higher than that in corresponding group (P <0.01). 68% of colorectal cancer cells have different degrees of AL-CAM staining. The expression of ALCAM was positively correlated with the depth of invasion, pTNM stage and lymph node metastasis. In CD24 and ALCAM positive cases, the expression of PCNA increased with the increase of CD24 expression (P <0.05). Colorectal cancer CD24 protein expression intensity and colorectal microvessel density was slightly correlated (r = 0.228, P = 0.019). The protein expression of ALCAM in colorectal cancer was not related to MVD (P = 0.17). Conclusion: CD24 and ALCAM may play an important role in the development of colorectal cancer.