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目的 评价基因重组乙型肝炎 (乙肝 )疫苗对人群的免疫效果。方法 对 195名新生儿、191名中学生及 2 19名不同HBV感染者接种疫苗 ,进行效果观察。并比较重组酵母来源和血源来源的 2种不同检测乙肝表面抗体的放射免疫分析方法试剂。结果 新生儿免疫后 ,抗 HBs阳转率均达到 85 %以上 ,但中华地鼠卵巢细胞基因重组乙肝疫苗不论是在抗体阳转率还是在抗体滴度上效果都优于酵母乙肝疫苗。中学生免疫后 ,抗 HBs阳转率达 70 %以上 ,免疫后 2年时 10 μg 5 μg 5 μg剂量组的免疫效果要优于 5 μg 5 μg 5 μg组。对HBV不同感染者免疫后 ,可使 5 1 14%的感染者抗 HBs阳转或抗 HBs滴度上升。重组酵母来源试剂检测抗 HBs的阳转率 (81 42 % )比血源来源试剂检测(73 33% )可提高近 10 %。结论 基因重组乙肝疫苗的免疫效果良好 ,对青少年接种疫苗可采用接种前不检测HBV抗体策略 ,以酵母乙肝疫苗 10 μg 5 μg 5 μg剂量为宜。在监测人群免疫后抗 HBs时 ,应选用相应抗原制备的试剂检测相应抗体 ,以提高检测的敏感性
Objective To evaluate the immune effect of gene recombinant hepatitis B (hepatitis B) vaccine on human population. Methods A total of 195 newborns, 191 middle school students and 2 19 HBV infected persons were vaccinated and the effect was observed. The two kinds of different reagents for the detection of hepatitis B surface antibody were compared between recombinant yeast source and blood source. Results After neonatal immunization, the anti-HBs positive rate reached more than 85%, but the Chinese hamster ovary gene recombinant hepatitis B vaccine is better than the yeast hepatitis B vaccine in terms of antibody positive rate or antibody titer. After immunization, the positive rate of anti-HBs was over 70%. After 2 years of immunization, the immunization effect of 10 μg 5 μg 5 μg dose group was better than that of 5 μg 5 μg 5 μg dose group. After immunization of different HBV infected persons, the titer of anti-HBs anti-HBs or anti-HBs could be increased in 51.14% of infected persons. The positive rate of anti-HBs detected by recombinant yeast-derived reagents (81 42%) can be increased by nearly 10% compared with that of blood-derived reagents (73 33%). Conclusions The recombinant hepatitis B vaccine has a good immunological effect. For vaccination of adolescents, the strategy of not detecting HBV antibody before vaccination can be adopted. Yeast hepatitis B vaccine 10 μg 5 μg 5 μg dose is appropriate. In monitoring the population of anti-HBs after immunization, the corresponding antigen preparation reagents should be used to detect the corresponding antibodies to improve the detection sensitivity