论文部分内容阅读
目的 了解中国 2 0 0 0～ 2 0 0 1年流行性感冒 (流感 )流行及抗原性变异情况。方法 鸡胚传代病毒用于抗原性分析 ;病毒液提取RNA进行逆转录 聚合酶链反应 (RT PCR) ,扩增产物纯化后测序。然后用MegAlign(Version1.0 3 )和Editseq(Version3 .69)软件进行基因种系发生树分析。结果 2 0 0 1年流行的H1N1亚型病毒血凝素蛋白重链 (HA1)区氨基酸序列与A 上海 7 99(H1N1)相比 ,在抗原决定簇D区的第 190位发生了氨基酸替换 ;基因种系发生树表明 2 0 0 1年的H1N1亚型流感病毒存在基因特性不同的两系病毒株。国内人群中仍然同时流行着两种抗原性明显不同的B型流感病毒 (Yamagata系和Victoria系 ) ,Yamagata系病毒占大多数 ,Victoria系的HA1区基因与B 山东 7 97毒株相比 ,其 197和 199位氨基酸发生了替换。B型的基因种系发生树也证实Victoria系病毒株的抗原性改变。 2 0 0 0年分离的H3N2亚型流感病毒的HA1区氨基酸序列与A 悉尼 5 97(H3N2 )间有 7～ 8个氨基酸的差异 ;2 0 0 1年分离的H3N2病毒株与 2 0 0 0年的病毒株相比 ,又在 83、186、2 0 2、2 2 2位发生了氨基酸替换 ,表明H3N2亚型病毒株间的抗原性发生了较明显的变异。结论 2 0 0 0～ 2 0 0 1年中国流感的流行情况较为平静 ;H3N2亚型的抗原性发生?
Objective To understand the prevalence and antigenic variation of influenza in China from 2000 to 2001. Methods Chicken embryo virus was used for antigenicity analysis. RNA was extracted from the virus solution for reverse transcription polymerase chain reaction (RT PCR). The amplified product was purified and sequenced. Then phylogenetic tree analysis was performed using MegAlign (Version 1.03) and Editseq (Version3.69) software. Results The amino acid sequence of the HA1 region of H1N1 subtype virus circulating in 2001 had amino acid substitution at position 190 in D region of antigenic determinant compared with that of Shanghai 799 (H1N1) The phylogenetic tree shows that two strains of viruses with different gene characteristics exist in the H1N1 subtype influenza virus in 2001. In the domestic population, two influenza B viruses (Yamagata strain and Victoria strain) with significantly different antigenicity are still prevailing at the same time. Yamagata virus accounts for the majority of the domestic population. The HA1 gene in Victoria strain, compared with B7 strain in Shandong province, The amino acids 197 and 199 have been replaced. The B-type gene phylogenetic tree also confirmed that the antigenicity of the Victoria strain was altered. The amino acid sequence of HA1 of H3N2 subtype influenza virus isolated in 2000 is 7-8 amino acids different from that of 597 (H3N2) in Sydney. H3N2 strain isolated in 2001 and 20000 Amino acid substitution occurred at 83,186,220,2222, indicating that the antigenicity between H3N2 subtypes was significantly different. Conclusion The epidemic situation of flu in China was relatively calm from 2000 to 2001. The antigenicity of H3N2 subtype was relatively stable.