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目的:观察IL-24在体外对非小细胞肺癌(non-small cell lung cancer, NSCLC)患者CD8n ＋T细胞功能的影响。n 方法:本研究入组28例NSCLC患者和17例健康对照者，收集外周血单个核细胞(peripheral blood mononuclear cells, PBMC)和支气管肺泡灌洗液(bronchoalveolar lavage fluid, BALF)，分选CD8n ＋T细胞，反转录实时定量PCR检测CD8n ＋T细胞中IL-24受体(IL-20R1、IL-20R2和IL-22R1)mRNA的相对表达量。不同浓度重组人IL-24(10 ng/ml和100 ng/ml)刺激纯化CD8n ＋T细胞后，流式细胞术检测穿孔素和颗粒酶B的表达变化。建立CD8n ＋T细胞和NSCLC细胞系NCI-H1882细胞的直接接触和间接接触体外共培养系统，观察IL-24刺激后CD8n ＋T细胞诱导靶细胞死亡比例，以及IFN-γ和TNF-α的表达变化。组间比较采用n t检验或LSD-n t检验。n 结果:CD8n ＋T细胞中未检测到IL-22R1 mRNA表达，CD8n ＋T细胞中IL-20R1和IL-20R2 mRNA相对表达量在健康对照者和NSCLC患者之间以及在非肿瘤部位和肿瘤部位之间的差异均无统计学意义(n P>0.05)。NSCLC患者外周血和肿瘤部位CD8n ＋T细胞中穿孔素和颗粒酶B水平显著低于健康对照者和非肿瘤部位(n P0.05)，而高浓度IL-24(100 ng/ml)则显著提升NSCLC患者CD8n ＋T细胞中穿孔素和颗粒酶B水平(n P0.05). Perforin and granzyme B expression was significantly reduced in CD8n + T cells from peripheral bloods and tumor sites of NSCLC patients as compared with those from healthy individuals and non-tumor sites (all n P0.05), but high concentration of IL-24 (100 ng/ml) significantly enhanced the expression of perforin and granzyme B in CD8n + T cells from NSCLC patients (n P<0.05). In the direct contact co-culture system, increased ratio of dead target cells and up-regulated IFN-γ and TNF-α expression were induced after stimulating CD8n + T cells from tumor sites in NSCLC patients with high concentration of IL-24 (100 ng/ml), but low concentration of IL-24 (10ng/ml) had no significant influence on CD8n + T cell-induced target cell death and cytokine production. In the indirect contact co-culture system, neither target cell death nor cytokine production induced by CD8n + T cells was affected by IL-24 stimulation.n Conclusions:High concentration of IL-24 promoted the n in vitro cytolytic function of CD8n + T cells from NSCLC patients, but might not influence the in vivo functions of CD8n + T cells.n