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目的:探讨口腔白斑及鳞癌染色体9p上4个微卫星位点改变的状况及其与临床病理诊断的关系。方法:选择微卫星位点D9S171、D9S175 2、D9S1748和IFNA ,应用聚合酶链式反应-变性聚丙烯酰胺凝胶电泳-银染方法,检测3 9例口腔白斑及12例鳞癌,分析其微卫星不稳定(MSI)及杂合性缺失(LOH)状况。结果:不同病理组别间4个位点MSI及LOH总的检出率有显著性差异(P <0 .0 1)。其中LOH检出率在不同临床病理组别之间有显著性差异(P <0 .0 5 ) ;而MSI的检出率在不同临床病理组别之间无显著性差异(P >0 .0 5 )。不同病理组别间单个位点的MSI及/或LOH无显著性差异(P >0 .0 5 )。结论:口腔癌的发生是多阶段多基因共同作用的结果。4个微卫星位点附近可能存在与口腔鳞癌发生发展相关的抑癌基因。MSI在口腔癌前病变癌变早期即已发生,而LOH发生频率则随口腔癌前病变癌变的发生发展逐渐增高
Objective: To investigate the changes of four microsatellite loci on chromosome 9p of oral leukoplakia and squamous cell carcinoma and their relationship with clinicopathological diagnosis. Methods: The microsatellite loci D9S171, D9S175 2, D9S1748 and IFNA were selected and 39 cases of oral leukoplakia and 12 cases of squamous cell carcinoma were detected by polymerase chain reaction-denaturing polyacrylamide gel electrophoresis-silver staining. Satellite instability (MSI) and loss of heterozygosity (LOH) status. Results: The overall detection rates of MSI and LOH were significantly different among 4 pathological groups (P <0. 01). Among them, the detection rate of LOH was significantly different between different clinicopathological groups (P <0.05), while the detection rate of MSI had no significant difference between different clinicopathological groups (P> 0.05) 5). There was no significant difference in MSI and / or LOH between single pathological groups (P> 0.05). Conclusions: Oral cancer is the result of multi-stage multi-gene interaction. There may be tumor suppressor genes associated with the occurrence and development of oral squamous cell carcinoma around 4 microsatellite loci. MSI in precancerous lesions of the oral mucosa has occurred earlier, while the frequency of LOH with oral precancerous lesions occurred gradually increased